Experimental studying of cerulloplasmin application for prevention of ischemically-reperfusion damage in cutaneous flap
Objective. To study in experiment a cerulloplasmin efficacy, applied for complete elimination and minimization of ischemically-reperfusion damage, causing necrosis of cutaneous flap (CF).
Маterials and methods. On 32 white male rats of the Sprague Dawley line with the body mass 250 g at average the investigations were conducted. The animals were divided into two Groups: Group I (control, n = 8) - intact animals; Group II (experimental, n = 24). Group II was divided into three Groups: IIА, IIБ and IIВ. In the animals of Group IIА cerulloplasmin was not applied and ischemia was not created, but the CF was not taken. In animals of Group IIБ cerulloplasmin also was not applied. To them 30 min before ischemia and with objective of its creation a serum was injected intraperitoneally. In 30 min after injection from two sides the clipses were put on epigastric veins and arteries for 10 h. In 10 h after initiation of ischemia in the flap the clipses were removed, аnd arterio-venous blood flow restored. Afterwards CF was sutured, using prolene threads, to its own place. Cerulloplasmin was injected intraperitoneally in dose 60 mg/kg to animals of Group IIВ 30 min before ischemia. After injection of cerulloplasmin the clipses were put on epigastric arteries and veins bilaterally, and in 10 h they were removed.
Results. Necrotic site of CF in Group IIА have constituted (1.8 ± 0.6) сm at average, IIБ - (22.4 ± 3.6) сm, and IIВ - (4.1 ± 1.1) сm.
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