Impact of the resection method on the structure–functional changes in pancreatic gland
Objective. To compare the changes in pancreatic tissues after еxperimental operative interventions, performed, using standard surgical technique and electric welding.
Маterials and methods. In experimental conditions a pancreatic resection was performed on laboratory animals – white rats, using standard surgical technique and electrocoagulator «Patommed ЕКVZ-300» in a welding regime. Pancreatic tissue was investigated immediately and in 3, 7 and 21 days after the operation.
Results. After resection, performed using standard surgical procedure, alternative changes have occurred, which were accompanied by circulatory disorders around foci of destruction in pancreatocytes, and the process progressing in a cascade-like character with formation of the colliquative necrosis foci. Partial restoration of functional state of parenchymatous cells with moderate reduction of a stromal signs of inflammation and microvascular reaction were observed on the 21-st day.
In the resection zone immediately after the electric welding conduction the tissue have had condensed due to the liquid compression and evaporation. But, on the 3-d and 7-th day a square of a necrotic parenchymatous damage due to thermal affection was lesser, then after application of a standard technique. The coagulation necrosis area, created as a result of a thermal impact, had reduced in size due to restoration by connective tissue and resorption by macrophages.
Conclusion. After applying of a standard surgical technique in early terms a more expressed inflammation have had occurred with a neutrophil infiltration predominance and more volume of a newly created connective tissue grown, than after electric welding, what might lead later to formation of cicatricial tissue in large volume. After the electric welding performance in a newly formatted tissue the ducts have evolved, what was not observed after usage of a standard surgical technique.
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