Impact of ulinastatin on metabolism of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 proteins in the patients, suffering an acute pancreatitis
Abstract
Objective. To investigate the impact on treatment of patients, suffering an acute pancreatitis (AP), using preparation ulinastatin (US) on functional state of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 protein (HMGB1).
Маterials and methods. Into the investigation 60 patients with an AP were included, who were treated in Department of Surgery № 2 of the Kyiv’s Municipal Clinical Hospital of Urgent Medical Aid. The indices of production of the oxygen reactive forms (ORF), which were determined by flowcytometry method, witnessed the state of the circulating phagocytes metabolism. Method of the immunoenzyme analysis was applied for determination of the HMGB1 level in serum.
Results. Propositive therapeutic effect of US have witnessed the lowering of the ORF synthesis by circulating leukocytes (twice at average), moderate strengthening of phagocytic activity of circulating mono- and polymorphonuclear phagocytes, lowering of the serum level of HMGB1 by 27%.
Conclusion. Inclusion of US into content of the treatment algorithm strengthens its anti-inflammatory and antioxidant effects in the patients, suffering an acute pancreatitis.
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