Impact of ulinastatin on metabolism of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 proteins in the patients, suffering an acute pancreatitis


Keywords: acute pancreatitis; ulinastatin; metabolism of leukocytes; nonhistonic High Mobility Group Box 1 proteins (HMGB1)

Abstract

Objective. To investigate the impact on treatment of patients, suffering an acute pancreatitis (AP), using preparation ulinastatin (US) on functional state of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 protein (HMGB1).

Маterials and methods. Into the investigation 60 patients with an AP were included, who were treated in Department of Surgery № 2 of the Kyiv’s Municipal Clinical Hospital of Urgent Medical Aid. The indices of production of the oxygen reactive forms (ORF), which were determined by flowcytometry method, witnessed the state of the circulating phagocytes metabolism. Method of the immunoenzyme analysis was applied for determination of the HMGB1 level in serum.

Results. Propositive therapeutic effect of US have witnessed the lowering of the ORF synthesis  by circulating leukocytes (twice at average), moderate strengthening of phagocytic activity of circulating mono- and polymorphonuclear phagocytes, lowering of the serum level of HMGB1 by 27%.

Conclusion. Inclusion of US into content of the treatment algorithm strengthens its anti-inflammatory and antioxidant effects in the patients, suffering an acute pancreatitis.

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Author Biographies


Ya. M. Susak, Bogomolets National Medical University named, Kyiv

Susak Yaroslav M. - MD, ScD (Med), professor,

Department of Surgery, Faculty of Dentistry

O.O. Bogomolet’s National Medical University

13, Taras Shevchenko boulevard, 01601, Kyiv, Ukraine

+38 067 233 39 65

yarsus@ukr.net

http://orcid.org/0000-0002-5102-485X


O. I. Zub, Kyiv’s Municipal Clinical Hospital of Urgent Medical Aid

Zub Oleksandr I. - surgeon, surgical department №2

City Clinical Emergency Hospital

3,Bratislavskaya street, 02000, Kyiv, Ukraine

+38 098 056 46 11

alexzubb1975@gmail.com

https://orcid.org/0000-0002-0276-1175


O. M. Lobanova, Kyiv’s Municipal Clinical Hospital of Urgent Medical Aid

Lobanova Oksana M. - surgeon, surgical department №2

City Clinical Emergency Hospital

3,Bratislavskaya street, 02000, Kyiv, Ukraine

+38 050 344 36 96

Oksana77lobanova@gmail.com

https://orcid.org/0000-0002-0332-0021


N. V. Senchylo, Іnstitute of Biology and Medicine of the Taras Shevchenko National University, Kyiv

Senchylo Natalia V. - PhD, associate professor

Department of Microbiology and Immunology

Institute of Biology and Medicine,

Taras Shevchenko National University of Kyiv

64/13, Volodymyrska Street, 01601, Kyiv, Ukraine

+38 099 059 10 44

natasha-bida@ukr.net

https://orcid.org/0000-0002-0879-615X


O. G. Fedorchuk, Kavetskyi Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv

Fedorchuk Oleksandr G. - PhD,

Senior Researcher,

Laboratory of Molecular and Cellular Metastasis Mechanisms

Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology,

45 Vasilkivska St., 03022, Kyiv, Ukraine

+38 097 324 88 15

fed6@mail.ru


L. M. Skivka, Іnstitute of Biology and Medicine of the Taras Shevchenko National University, Kyiv

Skivka Larysa M. - PhD, ScD (Biol), professor,

Head of the Department of Microbiology and Immunology

Institute of Biology and Medicine,

Taras Shevchenko National University of Kyiv

64/13, Volodymyrska Street, 01601, Kyiv, Ukraine

+38 067 998 80 64

realmed@ukr.net

https://orcid.org/0000-0002-2171-1085

Published
2018-10-29
How to Cite
Susak, Y. M., Zub, O. I., Lobanova, O. M., Senchylo, N. V., Fedorchuk, O. G., & Skivka, L. M. (2018). Impact of ulinastatin on metabolism of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 proteins in the patients, suffering an acute pancreatitis. Klinicheskaia Khirurgiia, 85(10), 21-25. https://doi.org/10.26779/2522-1396.2018.10.21
Section
General Problems of Surgery