Impact of ulinastatin on metabolism of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 proteins in the patients, suffering an acute pancreatitis
Keywords:
acute pancreatitis; ulinastatin; metabolism of leukocytes; nonhistonic High Mobility Group Box 1 proteins (HMGB1)
Abstract
Objective. To investigate the impact on treatment of patients, suffering an acute pancreatitis (AP), using preparation ulinastatin (US) on functional state of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 protein (HMGB1).
Маterials and methods. Into the investigation 60 patients with an AP were included, who were treated in Department of Surgery № 2 of the Kyiv’s Municipal Clinical Hospital of Urgent Medical Aid. The indices of production of the oxygen reactive forms (ORF), which were determined by flowcytometry method, witnessed the state of the circulating phagocytes metabolism. Method of the immunoenzyme analysis was applied for determination of the HMGB1 level in serum.
Results. Propositive therapeutic effect of US have witnessed the lowering of the ORF synthesis by circulating leukocytes (twice at average), moderate strengthening of phagocytic activity of circulating mono- and polymorphonuclear phagocytes, lowering of the serum level of HMGB1 by 27%.
Conclusion. Inclusion of US into content of the treatment algorithm strengthens its anti-inflammatory and antioxidant effects in the patients, suffering an acute pancreatitis.
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2. Atal SS, Atal S. Ulinastatin - a newer potential therapeutic option for multiple organ dysfunction syndrome. J Basic Clin Physiol Pharmacol. 2016; 27(2):91-9. doi: 10.1515/jbcpp-2015-0003.
3. Li D, Ji H, Zhao B, Xu C, Xia W, Han L, et al. Therapeutic effect of ulinastatin on pulmonary fibrosis via downregulation of TGFβ1, TNFα and NFκB. Mol Med Rep. 2018;17(1):1717-23. doi: 10.3892/mmr.2017.8056.
4. Pan Y, Fang H, Lu F. Ulinastatin ameliorates tissue damage of severe acute pancreatitis through modulating regulatory T cells. J Inflamm. (Lond) 2017;14:7. doi: 10.1186/s12950-017-0154-7.
5. Luo Y, Che W, Zhao M. Ulinastatin post-treatment attenuates lipopolysaccharide-induced acute lung injury in rats and human alveolar epithelial cells. Int J Mol Med. 2017;39(2): 297-306. doi: 10.3892/ijmm.2016.2828.
6. Yang R, Tenhunen J, Tonnessen TI. HMGB1 and Histones Play a Significant Role in Inducing Systemic Inflammation and Multiple Organ Dysfunctions in Severe Acute Pancreatitis. Int. J. Inflam. 2017:1817564. doi: 10.1155/2017/1817564.
7. Vourc'h M, Roquilly A, Asehnoune K. Trauma-Induced Damage-Associated Molecular Patterns-Mediated Remote Organ Injury and Immunosuppression in the Acutely Ill Patient. Front Immunol. 2018;9:1330. doi: 10.3389/fimmu.2018.01330.
8. Chan JK, Roth J, Oppenheim JJ, Tracey KJ, Vogl T, Feldmann M, et al. Alarmins: awaiting a clinical response. J Clin Invest. 2012;122(8):2711-19. doi: 10.1172/JCI62423.
9. Banks PA, Bollen TL, Dervenis C, Gooszen HG, Johnson CD, Sarr MG, et al. Acute Pancreatitis Classification Working Group. Classification of acute pancreatitis-2012: revision of the Atlanta classification and definitions by international consensus. Gut. 2013;62(1):102-11. doi: 10.1136/gutjnl-2012-302779.
10. Reiner NE, editor. Macrophages and dendritic cells. Methods and Protocols. NY, Humana Press; 2009: 368 s. P. DOI 10.1007/978-1-59745-396-7.
11. Rudyk MP, Pozur VV, Voieikova DO, Hurmach YV, Khranovska NM, Skachkova OV, et al. Sex-based differences in phagocyte metabolic profile in rats with monosodium glutamate-induced obesity. Sci Rep. 2018;8(1):5419. doi: 10.1038/s41598-018-23664-0.
12. Shrivastava P, Bhatia M. Essential role of monocytes and macrophages in the progression of acute pancreatitis. World J Gastroenterol. 2010;16(32):3995-4002. doi:10.3748/wjg.v16.i32.3995.
13. Susak YaM, Dyrda OO, Skivka LM, Fedorchuk OH, vynakhidnyky; Dyrda OO, patentovlasnyk. Sposib prohnozuvannia hniino-septychnykh uskladnen hostroho nekrotychnoho pankreatytu. Patent Ukrainy No 76812. 2013 sich 10.
14. Susak I, Skivka L, Tkachenko O, Khomenko I, Dyrda O. Assessment of circulating phagocyte functional state for the prediction of suppurative complications in patients with acute necrotizing pancreatitis. Pancreatology. 2015 June;15(3 Suppl): S6. doi: 10.1016/j.pan.2015.05.059.
15. Susak YaM, Dyrda OO, Skivka LM, Fedorchuk OH, vynakhidnyky; Dyrda OO, patentovlasnyk. Sposib prohnozuvannia efektyvnosti imunomoduliatora pry kompleksnomu likuvanni khvorykh na hostryi nekrotychnyi pankreatyt. Patent Ukrainy No 76813. 2013 sich 10.
16. Mirzaei S, Hadadi Z, Attar F, Mousavi SE, Zargar SS, Tajik A, et al. ROS-mediated heme degradation and cytotoxicity induced by iron nanoparticles: hemoglobin and lymphocyte cells as targets. J Biomo l Struct Dyn. 2017;18:1-11. doi: 10.1080/07391102.2017.1411832.
17. Oiva J, Mustonen H, Kylänpää ML, Kuuliala K, Siitonen S, Kemppainen E, et al. Patients with acute pancreatitis complicated by organ dysfunction show abnormal peripheral blood polymorphonuclear leukocyte signaling. Pancreatology. 2013;13(2):118-24. doi: 10.1016/j.pan.2013.01.010.
18. Song D, Song G, Niu Y, Song W, Wang J, Yu L, et al. Ulinastatin activates haem oxygenase 1 antioxidant pathway and attenuates allergic inflammation. Br J Pharmacol. 2014;171(19):4399-412. doi: 10.1111/bph.12780.
19. Bartel M, Hänsch GM, Giese T, Penzel R, Ceyhan G, Ketterer K, et al. Abnormal crosstalk between pancreatic acini and macrophages during the clearance of apoptotic cells in chronic pancreatitis. J Pathol. 2008;215(2):195-203. doi: 10.1002/path.2348.
Published
2018-10-29
How to Cite
Susak, Y. M., Zub, O. I., Lobanova, O. M., Senchylo, N. V., Fedorchuk, O. G., & Skivka, L. M. (2018). Impact of ulinastatin on metabolism of circulating leukocytes and the serum level of nonhistonic high mobility group box 1 proteins in the patients, suffering an acute pancreatitis. Klinicheskaia Khirurgiia, 85(10), 21-25. https://doi.org/10.26779/2522-1396.2018.10.21
Section
General Problems of Surgery
This work is licensed under a Creative Commons Attribution 4.0 International License.
