Impact of antiadhesive gels, manufactured on the base of sodium gyaluronate, on formation of intraperitoneal adhesions in experiment


Keywords: аntiadhesive gels; sodium hyaluronate; peritoneal adhesions; еxperiment

Abstract

Objective. To study the impact of antiadhesive gels (AAG), manufactured on base of sodium hyaluronate, on formation of intraperitoneal adhesions in experiment.

Маterials and methods. The investigation was conducted on 45 mature male and female rats of a Wistar line owing 200 – 250 gr body mass. On the first stage a simulation model of intraabdominal adhesions production was created, and on the second one – the impact of AAG, manufactured on the base of sodium hyaluronate, on intraabdominal adhesion-production in aseptic conditions and conditions of infectioning was studied.

Results. In laboratory animals, to which after creation of simulation model of intraabdominal adhesions production in conditions of infectioning a local application of concentrated gel (10 mg/ml), manifactured on the base of sodium hyaluronate, was accomplished a trustworthily more lower indices of the intraabdominal adhesion-production severity (р < 0.01) were observed in accordance to the estimation scales. Complete processing of abdominal cavity with AAG have had essentially prevented severity and spreading of postoperative peritoneal adhesions (р < 0.05). In presence of peritonitis the adhesive process severity was estimated at average in 2.14 points (0; 5) in accordance to Matoba scale, spreading – in 1.0 point (0; 2) in accordance to Hoffman scale, what also trustworthily lower than in comparison with a control group – accordingly 5,17 (3; 7) and 2,17 (2; 3) points.

Conclusion. Аpplication of gel, manufactured on base of sodium hyaluronate, in concentration 10 mg/ml on  focuses of peritoneal damage constitutes an effective method of prophylaxis for postoperative intraabdominal adhesion-production in experiment. The abdominal cavity irrigation with gel, based on the sodium hyaluronate gel in concentration 5 mg/ml, reduces a severity and prevalence of postoperative adhesion-production in aseptic conditions and in presence of peritonitis in experiment. Local application of gel, basing on sodium hyaluronate, in concentration 5 mg/ml do not cause a statistically significant inhibition of the adhesions production.

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Author Biographies


M. G. Melnychenko, Оdessa National Medical University

Melnychenko Maryna G. – MD, DSci (Med)., Full Professor,

Professor of the Department of Pediatric Surgery

Odessa National Medical University

Olhyivska street 4, Odessa, Odesa region, 65000, Ukraine.

Phone: +380501976185    

E-mail: marina_gm@i.ua                                           

ORCID iD: https://orcid.org/0000-0001-9066-4801


V. O. Sytnikova, Оdessa National Medical University

Sytnikova Varvara Aleksandrivna - MD, DSci (Med)., Full Professor,

Head of Pathomorphology Department

Odessa National Medical University

Olhyivska street 4, Odessa, Odesa region, 65000, Ukraine.

Phone: +380936711236     

E-mail: sitnvs@gmail.com      

ORCID iD: https://orcid.org/0000-0002-2111-669X

A. A. Kvashnina, Оdessa National Medical University

Kvashnina Anastasia A. - postgraduate of the Department

Department of Pediatric Surgery

Odessa National Medical University

Olhyivska street 4, Odessa, Odesa region, 65000, Ukraine.

Phone: + 380997246791      

E-mail: Kvashnina_Anastasia@i.ua 

ORCID iD: https://orcid.org/0000-0003-3704-2047

Published
2018-12-28
How to Cite
Melnychenko, M. G., Sytnikova, V. O., & Kvashnina, A. A. (2018). Impact of antiadhesive gels, manufactured on the base of sodium gyaluronate, on formation of intraperitoneal adhesions in experiment. Klinicheskaia Khirurgiia, 85(12), 64-67. https://doi.org/10.26779/2522-1396.2018.12.64
Section
Experimental Investigations