Impact of pharmacological correction on pathophysiological peculiarities of morpho-functional changes in lower urinary ways in experimental hyperactive bladder


Keywords: pharmacotherapy; hyperactive bladder; detrusor; restoration processes; sexual hormones

Abstract

Objective. Investigation of restorative processes in the lower urine-excreting ways under the influence of pharmacotherapy, and determination of the most perspective model of the experimental hyperactive bladder (HAB) treatment.

Маterials and methods. Еxperimental investigations were conducted on 120 sexually mature white male rats, in which a HAB was simulated. After creation of a hyperactive bladder (HAB) the laboratory animals were divided into four groups. Тhe laboratory animals of every one of three groups, except the fourth, were further divided into three subgroups, and in every subgroup pharmacocorrection was applied in various combinations.

Histological investigations of the bladder and urethra were conducted after exclusion of laboratory animals from the experiment in 14 and 28 days.

Results. The detrusor hyperactivity of the bladder have been lowered under the influence of pharmacotherapy, conducted in all the laboratory animals groups. In a group of laboratory animals, in which spasmex was applied, the signs of atony  were less pronounced, that in group of laboratory animals, in which mirabegron was injected. Міrabegron in combination with other preparations have lowered the detrusor tone in laboratory animals of all three subgroups significantly. Less pronounced signs of the detrusor atony, than in laboratory animals, in which mirabegron served as a background preparation, were observed in laboratory animals, to which testosterone and estradiol were injected. The detrusor tone was restored almost to the physiological norm level in all subgroups of laboratory animals, to which quercetin was injected with other preparations.

Conclusion. Spasmolytic preparations reduce the detrusor tone, but cause the bladder overstretching. Combination of quercetin with і статевими hormones, restorating the organ physiological state, have affected the bladder detrusor hyperactivity most favorably.

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Author Biographies


O. I. Yatsyna, National Cancer Institute, Kyiv

Yatsyna O.I., Ph.D., senior researcher,
National Cancer Institute
Lomonosova str 33/43 Kiev 03022, Ukraine
Phone: +380676985511
E-mail: yatsyna@gmail.com
ORCID: https://orcid.org/0000-0003-3081-9933


R. S. Vastyanov, Оdessa National Medical University

Vastyanov R.S. M.D., professor
Department of Pathological Physiology,
Odessa National Medical University,
Valikhnovskiy lane, 2, Odessa 65082, Ukraine
E-mail: rvastyanov@gmail.com
Phone: +38 0677347499
ORCID: https://orcid.org/0000-0001-8585-2517


I. M. Savytska, Shalimov National Institute of Surgery and Transplantology, Kyiv

Savytska I. M., Ph.D., Histologist, senior researcher,
Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680;
Phone: +380975100401
E-mail: irinasavitska@ukr.net
ORCID: https://orcid.org/0000-0002-2749-7825

 

S. V. Vernygorodskyi, National Pirogov Memorial Medical University, Vinnytsya

Vernygorodskyi S.V., professor
Department of Pathological Anatomy,
Vinnytsia National Pirogov Memorial Medical University
Pirogov Street, 56, Vinnytsia 21018, Ukraine
Phone: +380679565453
E-mail: vernset@ukr.net
ORCID: https://orcid.org/0000-0002-9314-8527


O. A. Geylenko, Shalimov National Institute of Surgery and Transplantology, Kyiv

Geylenko O.A., Histologist,
Shalimov Nacional Institute of Surgeri and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
ogeylenko@ukr.net
ORCID: https://orcid.org/0000-0001-5342-3881


F. I. Kostev, Оdessa National Medical University

Kostev F.I., M.D., professor
Department of Urology and Nephrology,
Odessa National Medical University,
Valikhnovskiy lane, 2, Odessa 65082, Ukraine
Phone: +38 048 723-33-24
E-mail: prof.kostev@gmail.com
ORCID: https://orcid.org/0000-0002-7625-9778

Published
2019-02-27
How to Cite
Yatsyna, O. I., Vastyanov, R. S., Savytska, I. M., Vernygorodskyi, S. V., Geylenko, O. A., & Kostev, F. I. (2019). Impact of pharmacological correction on pathophysiological peculiarities of morpho-functional changes in lower urinary ways in experimental hyperactive bladder. Klinicheskaia Khirurgiia, 86(2), 64-69. https://doi.org/10.26779/2522-1396.2019.02.64
Section
Experimental Investigations