Impact of multipotent stromal cells, аdsorped on polymer of lactic acid, on inflammatory reaction after its experimental implantation
Objective. To study the impact of autologous mesenchymal multipotent stromal cells (AMSC) of the bone marrow origin, аdsorped on polymer of lactic acid - polylactid (PL), on inflammatory process after experimental implantation of this polymer.
Маterials and methods. Using light microscopy with the help of luminescence the changes in subcutaneous-adipose cellular tissue in rats after implantation of PL with passively adsorped on surface of MMSC with a transfected gene GFP and additionally coloured with Vybrant® CM-Dil cellular membranes were studied.
Results. In one week near the PL implanted with adsorped MMSC, using methods of the fluorescent microscopy, the fibroblast-like cells were revealed with bright and smooth glow of cytoplasm while application of a rhodamine filter. On the second week near PL the macrophages of various size and form were present, with intense fluorescence of multiple inclusions in conditions of the rhodamine filter application. So on the luminescence brightness and the glowing objects quantity becomes progressively lowered up to almost complete elimination up to the fourth week. The sclerosed cellular tissue volume is reduced with raising of its vascularity on the 1 - 2-d weeks after implantation as a result of adsorption of AMSC on PL. In this cellular tissue during the first week the numeriсal density of all leucocytes and lymphocytes are lower. Тypical capsule around implanted PL is formed in rats up to the third week only. The capsule volume, its vascularization and cytogram of leucocytes did not depend from the MMSC adsorption.
Conclusion. While implantation of PL with adsorped MMSC already up to the second week these cellular elements from the tissues are eliminated by macrophages. AMSC and their detrit are eliminated completely from the introduction site up to the fourth week. Adsorption of AMSC on PL promotes reduction of the sclerosis severity and inflammatory changes in subcutaneous-adiposal cellular tissue in enhancement of its vascularization on the first-second weeks after the implantation. The terms of the capsule formation around PL, аs well as its structure, are not connected with application of cellular technologies.
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