Impact of multipotent stromal cells, аdsorped on polymer of lactic acid, on inflammatory reaction after its experimental implantation
Objective. To study the impact of autologous mesenchymal multipotent stromal cells (AMSC) of the bone marrow origin, аdsorped on polymer of lactic acid - polylactid (PL), on inflammatory process after experimental implantation of this polymer.
Маterials and methods. Using light microscopy with the help of luminescence the changes in subcutaneous-adipose cellular tissue in rats after implantation of PL with passively adsorped on surface of MMSC with a transfected gene GFP and additionally coloured with Vybrant® CM-Dil cellular membranes were studied.
Results. In one week near the PL implanted with adsorped MMSC, using methods of the fluorescent microscopy, the fibroblast-like cells were revealed with bright and smooth glow of cytoplasm while application of a rhodamine filter. On the second week near PL the macrophages of various size and form were present, with intense fluorescence of multiple inclusions in conditions of the rhodamine filter application. So on the luminescence brightness and the glowing objects quantity becomes progressively lowered up to almost complete elimination up to the fourth week. The sclerosed cellular tissue volume is reduced with raising of its vascularity on the 1 - 2-d weeks after implantation as a result of adsorption of AMSC on PL. In this cellular tissue during the first week the numeriсal density of all leucocytes and lymphocytes are lower. Тypical capsule around implanted PL is formed in rats up to the third week only. The capsule volume, its vascularization and cytogram of leucocytes did not depend from the MMSC adsorption.
Conclusion. While implantation of PL with adsorped MMSC already up to the second week these cellular elements from the tissues are eliminated by macrophages. AMSC and their detrit are eliminated completely from the introduction site up to the fourth week. Adsorption of AMSC on PL promotes reduction of the sclerosis severity and inflammatory changes in subcutaneous-adiposal cellular tissue in enhancement of its vascularization on the first-second weeks after the implantation. The terms of the capsule formation around PL, аs well as its structure, are not connected with application of cellular technologies.
2. Maiborodin IV, Kuznetsova IV, Beregovoy EA, Shevela AI, Maiborodina VI, Manaev AA, et al. Absence of complete resorption of polylactide material in the organism. Novosti Khirurgii. 2014;22(1): 26-32. doi: 10.18484/2305-0047.2014.1.26. [In Russian].
3. Shree N, Venkategowda S, Venkatranganna MV, Bhonde RR. Treatment with adipose derived mesenchymal stem cells and their conditioned media reverse carrageenan induced paw oedema in db/db mice. Biomed Pharmacother. 2017;90:350-3. doi: 10.1016/j.biopha.2017.03.090.
4. Qiu G, Zheng G, Ge M, Huang L, Tong H, Chen P, et al. Adipose-derived mesenchymal stem cells modulate CD14(++)CD16(+) expression on monocytes from sepsis patients in vitro via prostaglandin E2. Stem Cell Res Ther. 2017;8(1):97. doi: 10.1186/s13287-017-0546-x.
5. Tang XD, Shi L, Monsel A, Li XY, Zhu HL, Zhu YG, et al. Mesenchymal stem cell microvesicles attenuate acute lung injury in mice partly mediated by Ang-1 mRNA. Stem Cells. 2017;35(7):1849-59. doi: 10.1002/stem.2619.
6. Takeyama H, Mizushima T, Uemura M, Haraguchi N, Nishimura J, Hata T, et al. Adipose-derived stem cells ameliorate experimental murine colitis via tsp-1-dependent activation of latent TGF-?. Dig Dis Sci. 2017;62(8):1963-74 doi: 10.1007/s10620-017-4578-y.
7. Maiborodin IV, Morozov VV, Novikova JaV, Matveeva VA, Artemyeva LV, Matveev AL, et al. Acceleration of development of postoperative granulations after administration of mesenchymal stem cells near the thrombosed vein in experiment. Novosti Khirurgii. 2012;20(6):12-9. [In Russian].
8. Maiborodin IV, Matveeva VA, Maslov RV, Onoprienko NV, Kuznetsova IV, Chastikin GA. Fluorescent macrophages in the lymph nodes after application of multipotent mesenchymal stromal cells with transfected GFP gene. Novosti Khirurgii. 2014;22(5):526-32. doi: 10.18484/2305-0047.2014.5.526. [In Russian].
9. Maiborodin IV, Morozov VV, Anikeev АА, Figurenko NF, Maslov RV, Chastikin GA, et al. Macrophage reaction to multipotent mesenchymal stromal cells introduction into surgical trauma site in rats. Novosti Khirurgii. 2017;25(3):233-41. doi: 10.18484/2305-0047.2017.3.233. [In Russian].
10. Maiborodin IV, Maslov RV, Mikheeva TV, Elovskiy AA, Figurenko NF, Maiborodina VI, et al. The possibility of the angiogenesis in tissues remote from the place of the multipotent mesenchymal stromal cell injection. Molekulyarnaya meditsina. 2018;16(3):22-6. doi: 10.29296/24999490-2018-03-04. [In Russian].
11. Maiborodin IV, Maslov RV, Mikheeva TV, Elovskiy AA, Figurenko NF, Maiborodina VI, et al. Macrophagal adsorption of multipotent mesenchymal stromal cells and their debris from vascular bed proves the migration of these cellular elements through the vessels after tissue injection. Molekulyarnaya meditsina. 2018;16(3):56-61 doi: 10.29296/24999490-2018-04-10. [In Russian].
12. Krymskij LD, Nestajko GV, Rybalov AV. Rastrovaja jelektronnaja mikroskopija sosudov i krovi. Moskva: Medicina; 1976. 168 p. [In Russian].
13. Maiborodin IV, Elovskiy AA, Mikheeva TV, Figurenko NF, Maslov RV, Maiborodina VI, et al. The specific features of the inflammatory process accompanying ligation of the femoral vein in experiment under the conditions of stem cell therapy. Flebologiya. 2018;12(4):270-79. doi: 10.17116/flebo201812041270. [In Russian].
14. Head JR, Seeling LLJr. Lymphatic vessels in the uterine endometrium of virgin rats. J Reprod Immunol. 1984;6(3):157-66. PMID: 6737369.
15. Lee SK, Han CM, Park W, Kim IH, Joung YK, Han DK. Synergistically enhanced osteoconductivity and anti-inflammation of PLGA/?-TCP/Mg(OH)(2) composite for orthopedic applications. Mater Sci Eng C Mater Biol Appl. 2019;94:65-75. doi: 10.1016/j.msec.2018.09.011.
16. Fredriksson MI, Gustafsson AK, Bergstrom KG, Asman BE. Constitutionally hyperreactive neutrophils in periodontitis. J Periodontol. 2003;74(2):219-24. doi: 10.1902/jop.2003.74.2.219.
17. Li Y, Zhang W, Gao J, Liu J, Wang H, Li J, et al. Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway. Stem Cell Res Ther. 2016;7(1):102. doi: 10.1186/s13287-016-0356-6.
18. Stenger EO, Chinnadurai R, Yuan S, Garcia M, Arafat D, Gibson G, et al. Bone marrow-derived mesenchymal stromal cells from patients with sickle cell disease display intact functionality. Biol Blood Marrow Transplant. 2017;23(5):736-45. doi: 10.1016/j.bbmt.2017.01.081.
Copyright (c) 2019 Liga-Inform
This work is licensed under a Creative Commons Attribution 4.0 International License.