Implantation of the collagen membrane with adsorbed multipotent stem cells in experiment

Keywords: collagen; multipotent mesenchymal stem cells; implantation; аngiogenesis; macrophages

Abstract

Objective. To follow-up the processes, taking place with autologous multipotent mesenchymal stem cells (ММSC) of the bone marrow origin after their implantation into the organism on surface of the collagen material in experiment.

Маterials and methods. The changes in subcutaneous adipose cellular tissue in rats after implantation of the collagen membrane with passively adsorbed on the MMSC on the surface with transfected gene GFP and additionally stained cellular membranes with Vybrant® CM-Dil were studied up, using the light microscopy methods and a luminescence.

Results. While application of a luminescence in a one week after implantation of the collagen material with the MMSC adsorbed in a neighborhood with the implant there were revealed typical very small vessels owing a one-layer membrane, which have fluoresced intensely while a rodamine filter was installed. On the second week on periphery of the collagen material and in neighborhood to the implant on background of a rodamine filter application only small inclusions inside macrophages were shining with a red color. Subsequently step by step the quantity of such shining objects have reduced down to solitary ones, presenting very rarely, as well as their fluorescence intensity.

Conclusion. The main cause for the tissue vascularization enhancement around the implanted collagen with the MMSC adsorbed on the surface is their differentiation into cellular elements of vascular membranes. Starting from the second week all the MMSC undergo destruction and are phagocytized by macrophages, also in this term the structures from these MMSC disappear as a result of their differentiation, including the vessels.

Author Biographies

I. V. Маyborodin, Institute of Chemical Biology and Fundamental Medicine SD of RAS, Novosibirsk

Maiborodin Igor, MD, PhD, DrSci(Med), Professor,
Chief Researcher, Laboratory of Health Management Technologies,
Center of New Medical Technologies,
Institute of Chemical Biology and Fundamental Medicine,
Akademika Lavrenteva str., 8, Novosibirsk, 630090, Russian Federation
imai@mail.ru
http://orcid.org/0000-0002-8182-5084

S. V. Khomenyuk, Institute of Chemical Biology and Fundamental Medicine SD of RAS, Novosibirsk

Khomenyuk Sergey, MD, Junior Researcher,
Laboratory of Health Management Technologies,
Center of New Medical Technologies,
Institute of Chemical Biology and Fundamental Medicine,
Akademika Lavrenteva str., 8, Novosibirsk, 630090, Russian Federation
xoma774@ngs.ru
https://orcid.org/0000-0002-7346-926X

Т. V. Мikheyeva, Institute of Chemical Biology and Fundamental Medicine SD of RAS, Novosibirsk

Mikheeva Tatiana, MD, PhD, doctoral student
Laboratory of Health Management Technologies,
Center of New Medical Technologies,
Institute of Chemical Biology and Fundamental Medicine,
Akademika Lavrenteva str., 8, Novosibirsk, 630090, Russian Federation
tatiana.perrin@gmail.com
http://orcid.org/0000-0003-2249-5174

G. Yu. Yarin, Novosibirsk Scientific-Investigation Institute of Traumatology and Orthopedics

Gennadiy Yarin MD, PhD, Leading Researcher,
Functional Group of Neurovertebrology,
Novosibirsk Research Institute of Traumatology and Orthopaedics,
Frunze St. 17, Novosibirsk, 630091, Russian Federation
metrogyl@yandex.ru
https://orcid.org/0000-0003-2011-1253
Vitalina Maiborodina, MD, PhD, DrSci(Med),
Leading Researcher,
Laboratory of ultrastructural basis of pathology,
Institute of Molecular Pathology and Pathomorphology,
Akademika Timakva str., 2, Novosibirsk, 630117, Russian Federation
imai@mail.ru
http://orcid.org/0000-0002-5169-6373

N. V. Оnopriyenko, Novosibirsk State Medical University

Onoprienko Natalya, MD, PhD, DrSci(Med), Assistant
Department of Obstetrics and Gynecology, Faculty of Medicine,
Novosibirsk State Medical University,
Krasny Prospect 52, Novosibirsk, 630091, Russian Federation
nata.onoprienko@gmail.com
http://orcid.org/0000-0002-5657-1676

I. А. Vilgelmi, Novosibirsk Scientific-Investigation Institute of Traumatology and Orthopedics

Vilgelmi Inna, researcher
Functional Group of Neurovertebrology,
Novosibirsk Research Institute of Traumatology and Orthopaedics,
Frunze St. 17, Novosibirsk, 630091, Russian Federation
dr.vilgelmi.allsurg@yandex.ru
https://orcid.org/0000-0001-7769-6147

А. I. Shevela, Institute of Chemical Biology and Fundamental Medicine SD of RAS, Novosibirsk

Shevela Andrew, MD, PhD, DrSci(Med), Professor,
Head of the Center of New Medical Technologies,
Institute of Chemical Biology and Fundamental Medicine, T
Akademika Lavrenteva str., 8, Novosibirsk, 630090, Russian Federation
ashevela@mail.ru
http://orcid.org/0000-0002-3164-9377

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Published
2019-06-27
How to Cite
МаyborodinI. V., Khomenyuk, S. V., МikheyevaТ. V., Yarin, G. Y., МаyborodinaV. I., ОnopriyenkoN. V., VilgelmiI. А., & ShevelaА. I. (2019). Implantation of the collagen membrane with adsorbed multipotent stem cells in experiment . Klinicheskaia Khirurgiia, 86(6), 53-59. https://doi.org/10.26779/2522-1396.2019.06.53
Section
Experimental Investigations