The impact of partial obstruction of common biliary duct without hyperbilirubinemia on the liver

Keywords: partial obstruction of common biliary duct; cholestasis; morphology of the liver; hyperbilirubinemia

Abstract

Objective. Experimental studying of hepatic morphological changes and the blood biochemical indices in partial obstruction of common biliary duct (POCBD) without hyperbilirubinemia.

Materials and methods. POCBD was simulated on 34 laboratory animals. The animals were excluded from the experiment on the 3, 7, 14, 21, 28-th and 35-th day. The content of bilirubin, albumin, the blood serum urea and activity of alanine aminotransferase (AlAT), aspartate aminotransferase (AsAT), alkaline phosphatase (APH) were measured. The common biliary duct diameter, hepatic volume and mass were measured, hepatic histology investigated, and morphometry was performed. Control group consisted of 17 experimental animals.

Results. In 14 (41.2%) experimental animals POCBD without hyperbilirubinemia was observed. Maximal morphological changes were noted on the 28 - 35th day: the hepatic mass enhancement by 31% and the hepatic volume - by 25.5%; increase of the number and dilatation of biliary ducts, increase of the sinusoidal cells quantity, of the hepatocytes square, presence of the Degree II in 4of 5 observations. The changes of biochemical indices were trustworthy on the 3 - 7-th day: the albumin content have lowered down to (21.6 ± 2.5) g/l, while a control index constitutes (26.7 ± 1.6) g/l. Activity of AlAT, AsAT, APH, the urea content during the experiment did not differ from the control values.

Conclusion. Despite a complete functional compensation, POCBD without hyperbilirubinemia leads to significant morphological changes in the live, in spite of complete functional compensation presence.

Author Biographies

I. N. Mamontov, Kharkov Medical Academy of Postgraduate Education

Mamontov Ivan Mikolayovich, MD, PhD(Med), Assistant Professor
department of surgery and proctology
Kharkiv Medical Academy of Postgraduate Education,
+38 (057) 7601166
ivan.n.mamontov@gmail.com
ORCID ID: http://orcid.org/0000-0003-0059-2715

T. I. Tamm, Kharkov Medical Academy of Postgraduate Education

Tamm Tamara Ivanivna, MD, DSci(Med), Professor
Chief of the department of surgery and proctology
Kharkiv Medical Academy of Postgraduate Education,
61037, Kharkiv, pr. Moskovsky, 197
+38 (057) 7601166
tamm_ti@ukr.net
ORCID ID: http://orcid.org/0000-0001-6372-2092

I. V. Ivakhno, Kharkov Medical Academy of Postgraduate Education

Ivakhno Igor Volodymyrovych, MD, PhD(Med), Teaching Assistant, doctor-pathologist
department of pathology
Kharkiv City Clinical Hospital No17,
Kharkiv Medical Academy of Postgraduate Education,
61037, Kharkiv, pr. Moskovsky, 195
Tel.: +38 (057) 7252482
E-mail igorv.ivakhno@gmail.com
ORCID ID: http://orcid.org/0000-0002-5229-0068

V. A. Panasenko, Kharkov National Medical University

Panasenko V’yacheslav Oleksyyovych, MD, Teaching Assistant
Department of Histology, Cytology, and Embryology.
Kharkiv National Medical University,
Nauky Avenue, 4, Kharkiv 61022, Ukraine
+38 (057) 707-73-46
v.o.pan@ukr.net
ORCID ID: http://orcid.org/0000-0002-2803-7994

V. I. Padalko, Karazin Kharkov National University

Padalko Volodymyr Illich, PhD(Biology), Senior Research Worker, Associate Professor
Department of General and Clinical Pathology
Karasin Kharkiv National University, the School of Medicine,
+38 (057) 707-55-00
padalko@karazin.ua
ORCID ID: http://orcid.org/0000-0002-2690-8995

I. I. Zulfugarov, Kharkov Medical Academy of Postgraduate Education

Ismat Zulfugarov, Graduate student
department of surgery and proctology
Kharkiv Medical Academy of Postgraduate Education,
61037, Kharkiv, pr. Moskovsky, 197
+38 (057) 7601166
abramovmatvey26@gmail.com
ORCID ID: http://orcid.org/0000-0003-1007-7493

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Published
2019-08-29
How to Cite
Mamontov, I. N., Tamm, T. I., Ivakhno, I. V., Panasenko, V. A., Padalko, V. I., & Zulfugarov, I. I. (2019). The impact of partial obstruction of common biliary duct without hyperbilirubinemia on the liver. Klinicheskaia Khirurgiia, 86(8), 67-71. https://doi.org/10.26779/2522-1396.2019.08.67
Section
Experimental Investigations