Morphologic characteristic of the lungs in the patients, suffering experimental peritonitis

Keywords: acute extended peritonitis; lungs; morphological changes

Abstract

Objective. To study and estimate the morphological changes in the lungs of laboratory animals with experimental acute extended peritonitis.

Materials and methods. In this investigation were applied on 32 white laboratory rats. An acute peritonitis was simulated, using injection of 0.5 ml of a 10% solution of the fecal suspension probed into abdominal cavity of laboratory animals. The observation terms were implemented on the first, third and the seventh day from the beginning of the peritonitis simulation. Pulmonary tissue was taken for histological investigation. The organ pieces obtained were fixated in 10% solution of neutral formalin and then colored using hematoxylin and eosin.

Results. Distelectasis, the sites of collapse and emphysematous dilation of alveoli, have had occurred during all observation period of the exudative-inflammatory vascular reaction. The bronchi wall have thickened predominantly due to cellular infiltrates, the bronchioles lumens were free of exudate and cellular elements.

Conclusion. The sites of collapsing and emphysematous dilation of alveoli were revealed during histological investigation of pulmonary tissues in experimental animals with simulated acute extended peritonitis, comparing with intact rats in the first day of the experiment. On the third day the majority of vessels have had wide lumens filled with the blood, and erythrodiapedesis occurred into perivascular compartments and into lumens of a separate alveoli. On the 7th day significant dilation of the veins and the arteries filling with the blood was noted.

Author Biographies

I. Ya. Dzubanovsky, I. Gorbachevskyi Ternopil National Medical University

Dzubanovsky I. Ya. - MD, DSci(Med), Professor,
Head of Department of Surgery
Institute of Postgraduate Education
Kupchynskogo, 14, Ternopil, 46003;
I. Horbachevsky Ternopil National Medical University;
dzubanovsky@tdmu.edu.ua;
ORCID ID: http://orcid.org/0000-0001-8852-3938.

B. M. Vervega, Danylo Galytskyi Lviv National Medical University

Vervega B. M. - MD, PhD, Assistant of Professor
Department of Pathological Physiology
Danylo Halytsky Lviv National University
+380677186312.

S. R. Pidruchna, I. Gorbachevskyi Ternopil National Medical University

Pidruchna S. R. - MD, DSci(Med),
Head of Department of Medical Biochemistry
I. Horbachevsky Ternopil National Medical University;
m.Voli, 1, Ternopil, 46001;
pidruchna@tdmu.edu.ua;
+380986408627;
ORCID ID: http://orcid.org/0000-0002-5607-5680.

N. A. Melnyk, I. Gorbachevskyi Ternopil National Medical University

Melnyk N. A. - MD, PhD, Assistant
Department of General Hygiene and Ecology
I. Horbachevsky Ternopil National Medical University;
m.Voli, 1, Ternopil, 46001;
melnyknan@tdmu.edu.ua;
+380971858204;
ORCID ID: http://orcid.org/0000-0002-7357-7551.

References

Bajaj JS, Tandon P, O’leary J, Wong F, Biggins S, Garcia-Tsao G, et al. Primary spontaneous bacterial peritonitis prophylaxis is associated with greater ICU admission and 30-day mortality compared to secondary spontaneous bacterial peritonitis prophylaxis. J Hepatol. 2018;68(Suppl 1):65-104. doi: 10.1016/S0168-8278(18)30353-2.

Enomoto H, Inoue Sh-i, Matsuhisa A, Nishiguchi Sh. Diagnosis of Spontaneous Bacterial Peritonitis and an In Situ Hybridization Approach to Detect an “Unidentified” Pathogen. [Internet]. Int J Hepatol. 2014;4:1-8. Available from: https://www.hindawi.com/journals/ijh/2014/634617/cta/ doi: 10.1155/2014/634617.

Yang SK, Xiao L, Zhang H, Xu XX, Song PA, Liu FY, Sun L. Diagnostic Accuracy of Serum Procalcitonin for Spontaneous Bacterial Peritonitis Due to End-stage Liver Disease: A Meta-analysis. BMC Infect Dis. 2014;14:452. doi: 10.1186/1471-2334-14-452.

Dzubanovsky IYa, Mignenko BO, Polyatsko KG. [Poliorhanna dysfunkciia u xvorykh na hostryi poshyrenyi perytonit]. Kharkivska khirurhichna shkola. 2007;4:70-3. [In Ukrainian].

Milić L, Grigorov I, Krstić S, Ćeranić MS, Jovanović B, Stevanović J, et al. Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status. J. Med. Biochem. 2017;36(1):44-53. doi: 10.1515/jomb-2016-0016.

Gerasymchuk MR, Zayats LM, Cherkasova VV. The role of endogenous intoxication and neutrophils in mechanisms of acute lung injury in case of experimental peritonitis. Vestnyk KazNMU. 2013;5:76-80. [In Ukrainian].

Gerasymchuk MR, Klishch IP, Varnava NYa. Place of endogenous intoxication at an early stage of development of the critical states of various etiologies. Inter-Medical. 2015;3(9):21-4.

Gerasymchuk MR, Zayacz LM, Cherkasova VV. Vplyv polietiolohichny`x eksperymentalnykh staniv na funkcionalnyi stan legen. Eksperymentalna i klinichna medycyna. 2016;(2):44-8. [In Ukrainian].

Mujahid S, Nielsen HC, Volpe MV. MiR-221 and miR-130a Regulate Lung Airway and Vascular Development. PLoS ONE. 2013;8(2):55-9. doi: 10.1371/journal.pone.0055911.

Karayama M, Inui N, Mori K, Kono M, Hozumi H, Suzuki Y, et al. Respiratory impedance is correlated with morphological changes in the lungs on three-dimensional CT in patients with COPD. Sci Rep. 2017;7:41709. doi: 10.1038/srep41709.

Ribeiro Júnior G, de Souza Xavier Costa N, Belotti L, Dos Santos Alemany AA, Amato-Lourenço LF, da Cunha PG, et al. Diesel exhaust exposure intensifies inflammatory and structural changes associated with lung aging in mice. Ecotoxicol Environ Saf. 2019;170:314-23. doi:10.1016/j.ecoenv.2018.11.139.

Hack CE, Gearhart JM. A rabbit lung morphology model for aerosol deposition and clearance. J Aerosol Science. 2016;99:144-56. doi:10.1016/j.jaerosci.2016.03.004.

Pidruchna SR. Dynamics of changes of the endogenous intoxication indices in the animals, suffering severe and combined trauma, after correction, using xenotransplants. Klin khir. 2013;(2):71-4. PMID: 23705489.

Pidruchna SR. Impact of xenoimplants on metabolic changes in the liver of rats in combined trauma. Klin khir. 2013;(3):59-62. PMID: 23718038.

Lazarenko VA, Lypatov VA, Blynkov YuYu, Skorykov DV. Eksperymentalnaia model rasprostranennoho kalovoho perytonyta. Chelovek y eho zdorove. 2008;4:128-32. [In Russian].

Logoyda L, Kondratova Y, Korobko D, Susla O, Soroka Yu, Tsytsiura R, et al. Youden’s test of the chromatographic determination of captopril in pharmaceuticals. Int J Green Pharm. 2017;11(3):188-91. doi: 10.22377/ijgp.v11i03.1124.

Published
2019-08-29
How to Cite
Dzubanovsky, I. Y., Vervega, B. M., Pidruchna, S. R., & Melnyk, N. A. (2019). Morphologic characteristic of the lungs in the patients, suffering experimental peritonitis. Klinicheskaia Khirurgiia, 86(8), 72-75. https://doi.org/10.26779/2522-1396.2019.08.72
Section
Experimental Investigations