Application of microRNA-34a for diagnosis of pleomorphic adenomas of salivary glands

I. S.  Brodetskyi; V. O.  Malanchuk; V. E.  Dosenko

doi:10.26779/2522-1396.2019.10.67

I. S. Brodetskyi Bogomolets National Medical University, Kyiv https://orcid.org/0000-0002-9434-4079
V. O. Malanchuk Bogomolets National Medical University, Kyiv http://orcid.org/0000-0001-8111-0436
V. E. Dosenko Bogomolets Institute of Physiology, Kyiv http://orcid.org/0000-0002-6919-7724

DOI: https://doi.org/10.26779/2522-1396.2019.10.67

Keywords: pleomorphic adenomas; genetic investigations; micrRNA-34a; apoptosis

Abstract

Objective. Determination of expression of microRNA-34a in tissues of pleomorphic adenomas of large salivary glands, adjacent to tumor salivary gland tissue, intact tissue of a salivary gland, which was not connected with the tumor, and in a venous blood as well.

Materials and methods. The investigation was conducted in 20 patients, suffering benign tumors of large salivary glands (pleomorphic adenomas). Expression was estimated, using adverse transcription and quantitative polymerase chain reaction in regime of a real time.

Results. Analysis of the expression level for microRNA-34a was conducted in the tumoral tissue, adjacent to the tumor salivary gland tissue, and in intact tissue of salivary gland, which lacked a link with the tumor, the venous blood in patients, suffering pleomorphic adenomas of large salivary glands, and there was revealed, that it have appeared the highest in the salivary gland adjacent to the tumor - (1052.02 ± 367.20, comparing with the same index in the intact gland - 47.72 ± 28.93).

Conclusion. Level of expression of microRNA-34a in the tissues of pleomorphic adenomas of salivary glands, which is in 11 times higher, than in norm (in intact tissue of salivary gland), may be applied as a genetic marker for verification of these tumors.

Author Biographies

I. S. Brodetskyi, Bogomolets National Medical University, Kyiv

Brodetskyi Igor S. - PhD(Med), Associate Professor
Department Oral and Maxillofacial surgery
Bogomolets National Medical University,
Blvd. Shevchenko 13, 01601, Kyiv, Ukraine
+380632035339
i2g3o4@bigmir.net
Ідентифікатор ORCID https://orcid.org/0000-0002-9434-4079

V. O. Malanchuk, Bogomolets National Medical University, Kyiv

Malanchuk Vladislav A. - DSci(Med), Professor,
Head of the Department Oral and Maxillofacial surgery
Bogomolets National Medical University,
Blvd. Shevchenko 13, 01601, Kyiv, Ukraine
+380965533425
Ідентифікатор ORCID http://orcid.org/0000-0001-8111-0436

V. E. Dosenko, Bogomolets Institute of Physiology, Kyiv

Dosenko Victor E. - DSci(Med), Professor,
Head of the Department of General and Molecular Pathophysiology,
Bogomolets Physiology Institute
01024, Kyiv, Ukraine,
+380442562481
dosenko@biph.kiev.ua
Ідентифікатор ORCID http://orcid.org/0000-0002-6919-7724

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