Impact of the impulse electrophoresis of the blood plasma on the wounds healing and formation of cicatricial tissue in experiment

Keywords: postoperative cicatrix; the impulse electrophoresis; regeneration of tissues; creation of collagen

Abstract

Objective. To study in experiment the impact of the impulse electrophoresis of the blood plasma method on the wounds healing process in postoperative wounds.

Materials and methods. The investigations were conducted on 15 white rats, to which in aseptic environment under intraabdominal narcosis the hip surgical access through the skin and muscles was conducted with subsequent layered closure of the wound, using separate knots. Ten laboratory animals were included into the main group, in whom transdermal introduction of the blood plasma, using the impulse electrophoresis method, was conducted on the 4th, 7th, 10th and 14th postoperative days. Into a control group 5 white rats were included, in which transdermal introduction of the blood plasma was not applied postoperatively. In all the laboratory animals the wounds have healed primarily. On the19th day postoperatively the laboratory animals were extracted from the experiment, and the near suture skin was investigated, using histological methods.

Results. The surgical access healing in the hip skin and muscles with formation of normotrophic cicatrices was observed in the main group of the laboratory animals. Hypertrophic cicatrix in the operative intervention zone have been formatted in the laboratory animals of a control group on the 19th postoperative day.

Conclusion. More qualitative healing of the wounds with formation of normotrophic cicatrix while application of transdermal introduction of the blood plasma, using the impulse electrophoresis method, was confirmed in experimental investigation in laboratory animals of the main group.

Excessive creation of collagen was omitted in the laboratory animals of the main group, what have promoted formation of more thin and tender cicatrix, which have become slightly visible up to the end of experiment.

Author Biographies

G. V. Terehov, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Terehov GV, MD, PhD(Med),
Head of the Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
doctorterehov@gmail.com;
0679311431
orcid.org/ 000-0002-7625-9778

M. V. Chukhraiev, Scientific-Methodic Centre «MEDINTECH» Ltd, Kyiv

Chukhraiev MV., MD, PhD(Med), Professor, DSci(Medical Psychology),
Head of MEDINTECH (Kiev)
E-mail: nv1111155@gmail.com;
office 2, Pochayninska 23 str., Kyiv, Ukraine, 04070,
Tel. 0674400304
orcid.org/ 0000-0001-5290-8887

M. V. Kostyliev, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Kostyliev M.V. MD, DSci(Med),
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
E-mail mkv.via@gmail.com;
orcid.org/0000-0002-2559-4058

S. P. Galych, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Galych S.P. MD, DSci(Med), Professor
Chief of department of microsurgery & plastic surgery,
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
E-mail galych.sergey@gmail.com,
Teл. 0444550608;
orcid.org/0000-0001-6179-5992

O. Yu. Furmanov, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Furmanov O.Yu. MD, DSci(Med), senior research
department of microsurgery & plastic surgery,
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
E-mail 777doc.doc@gmail.com
0000-0001-7042-6080

I. M. Savytska, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Savytska IM, MD, PhD(Med), senior researcher,
Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantation
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
E-mail - irinasavitska@ukr.net
0975100401.
orcid.org/ 0000-00020-2749-7825

O. A. Geylenko, O. O. Shalimov National Institute of Surgery and Transplantology, Kyiv

Geylenko OA., researcher,
Shalimov National Institute of Surgery and Transplantation
E-mail - ogeylenko@ukr.net.
30, Geroyev Sevastopolya str., Kyiv, Ukraine, 03680
Tel. 0667181191.
orcid.org/ 0000-0001-5342-3881

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Chukhraev MV, Terehov GV, Dmitrenko IP, vinahidnyky; Chukhraev MV, Terehov GV, Dmitrenko IP, patentovlasnyky. Sposib likuvannya psoriatichnogo urazhennya. Patent Ukrainy No 123508. 2018 Lut 26. [In Ukrainian].

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Published
2019-12-20
How to Cite
Terehov, G. V., Chukhraiev, M. V., Kostyliev, M. V., Galych, S. P., Furmanov, O. Y., Savytska, I. M., & Geylenko, O. A. (2019). Impact of the impulse electrophoresis of the blood plasma on the wounds healing and formation of cicatricial tissue in experiment . Klinicheskaia Khirurgiia, 86(11-12), 69-71. https://doi.org/10.26779/2522-1396.2019.11-12.69
Section
Experimental Investigations