Studying of capacity of the inflamed intestinal wall to accumulate tienam preparation in experiment

Keywords: accumulation of antibacterial preparations in intestinal wall; tienam; experimental mechanical ileus

Abstract

Objective. Using experimental pattern of mechanical ileus, to study the capacity of nonchanged and inflamed intestine to accumulate a tienam preparation.

Materials and methods. The experiment was conducted on 60 laboratory animals in two stages. On the first stage the content of tienam in the blood, parenchymatous organs and in the nonchanged intestinal wall was determined. On the second stage the content of the tienam components was determined in the blood, parenchymatous organs and in the wall of inflamed suprastenotic part of intestine. In both series the preparation was injected intravenously in accordance to scheme, calculated for the animals body mass. The quantity of tienam preparation in the investigated material was determined, using the method of a highly effective liquid chomatography.

Results. The experimental investigation conducted have shown, that the tienam preparation components in nonoperated animals are accumulated in therapeutic doses in the blood, liver and kidneys. In the animals, in whom mechanical ileus was simulated, he tienam components were revealed only in parenchymatous organs and in the blood. In the inflamed intestinal wall the tienam components were not revealed even while injection of a dose, prevailing a middle therapeutic one  in 2.5 times.

Conclusion. Imipenem and cilastatin, which are included into tienam preparation, while secondary injections in a dose, prevailing a middle therapeutic one  in 2.5 times, were not revealed in the wall of the inflamed intestine in experimental mechanical ileus.

Author Biography

V. V. Nepomniashchyi, Kharkov Medical Academy of Postgraduate Education

Nepomniashchyi Valentin Vladimirovich, PhD (Med), Assistant professor
Department of surgery and proctology
Kharkiv Medical Academy of Postgraduate Education,
61037, Kharkiv, 197, Moskovsky ave,
+38 (057) 7601166
doktor.nep@gmail.com
ORCID ID 0000-0001-6262-6795

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Published
2019-12-20
How to Cite
Nepomniashchyi, V. V. (2019). Studying of capacity of the inflamed intestinal wall to accumulate tienam preparation in experiment. Klinicheskaia Khirurgiia, 86(11-12), 72-77. https://doi.org/10.26779/2522-1396.2019.11-12.72
Section
Experimental Investigations

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