Comparative studying of biochemical indices of the blood in experimental acute biliary pancreatitis and obstructive cholestasis
Objective. Comparative studying of levels of some biochemical indices in experimental acute biliary pancreatitis and obstructive cholestasis.
Materials and methods. An acute biliary pancreatitis was simulated on 32 rats and obstructive cholestasis – on 23. On the first, third and 7th days after the blood indices of general bilirubin, albumin and the urea, as well as activity of alaninaminotranspherase, aspartataminotranspherase, alkaline phosphatase and amylase were studied.
Results. In the acute biliary pancreatitis on the second day of the experiment 10 (31.3%) rats have deceased, and in obstructive cholestasis - 1 (4.3%) rat (p < 0.01). In both groups of laboratory animals the signs of cholestasis were determined, and in the acute biliary pancreatitis – signs of pancreatitis. On the first day in acute biliary pancreatitis in comparison with acute cholestasis there were noted the enhanced values of the amylase activity (p < 0.001) and the urea content (p < 0.05); on the third day – the lower values of the general bilirubin content (p < 0.001), higher values of the alaninaminotranspherase activity (p < 0.05), the urea content (p < 0.01) and activity of amylase (p < 0.05); on the 7-th day – lower values of general bilirubin (p < 0.05), higher values of activity of alkaline phosphatase and aspartataminotranspherase (p < 0.05).
Conclusion. More severe hyperbilirubinemia is observed in obstructive cholestasis. In the acute biliary pancreatitis the trustworthy enhanced values of the alaninaminotranspherase (on the third day) and aspartataminotranspherase (on the 7-th day) activities, the urea content (on the third day), as well as activity of alkaline phosphatase (on the 7th day) are noted.
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