Complex treatment of experimental purulent wound of soft tissues

Keywords: еstrogens; liposomes; purulent wound.

Abstract

Objective. To study the impact of estrogens and liposomes on course of healing process in purulent wounds of soft tissues in experiment.

Маterials and methods. Еxperimental investigation was done on 21 sheep. In the laboratory animals purulent-inflammatory process was simulated. Тhe laboratory animals were distributed into two groups: a control - 6 sheep and investigated - 15. All the animals obtained conventional therapy. In laboratory animals of the investigated group estrogens and liposomes were added to complex of the conventional treatment measures. The healing process course was controlled, using cytological, biochemical, planimetric and mathematic-statistical methods of investigation.

Results. Application of combined estrogen-liposomal therapy in complex treatment of experimental purulent wound of soft tissues have enhanced the levels of оxyprolene, ribonucleic and deoxyribonucleic acids in the wound area, raised a value of estradiol/testosteronic index, accelerated the wound cleansing, the granulations creation and shortened the wound healing process by (2.12 ± 0.24) days.

Conclusion. Including of estrogens and liposomes into the treatment complex for experimental purulent wound of soft tissues enhances cellular biosynthetic activity, activity of reparative processes in the wound area, accelerates the wound cleansing and the granulation creation, shortens the wound healing process and may be applied for improvement of the purulent wounds treatment in elderly and senile patients.

Author Biographies

О. V. Lihonenko, Ukrainian Medical Stomatological Academy, Poltava

Lihonenko Oleksii V. MD, DSci(Med), professor
Head of the Department of General Surgery
Ukrainian Medical Stomatological Academy.
36011 Ukraine, Poltava, Shevchenka Str. 23,
+38(05322)2-68-74,
oligonenko@gmail.com
http://orcid.org/0000-0002-2772-2244

О. V. Storozhenko, Ukrainian Medical Stomatological Academy, Poltava

Storozhenko Oleksii V. MD, PhD, assistant professor,
Department of General Surgery,
Ukrainian Medical Stomatological Academy.
36011 Ukraine, Poltava, Shevchenka Str. 23,
+38(05322)2-68-74,
storozh75@gmail.com
http://orcid.org/0000-0003-4061-1650

А. B. Zubakha, Ukrainian Medical Stomatological Academy, Poltava

Zubakha Anatolii B. MD, PhD, assistant professor,
Department of General Surgery,
Ukrainian Medical Stomatological Academy.
36011 Ukraine, Poltava, Shevchenka Str. 23,
+38(05322)2-68-74,
zubacha@gmail.com
http://orcid.org/0000-0002-2528-6805

І. А. Shumeiko, Ukrainian Medical Stomatological Academy, Poltava

Shumeiko Ihor A. MD, PhD, assistant professor,
Department of General Surgery,
Ukrainian Medical Stomatological Academy.
36011 Ukraine, Poltava, Shevchenka Str. 23,
+38(05322)2-68-74,
doctorfender@mail.ru
http://orcid.org/0000-0003-0755-3073

І. О. Chorna, Ukrainian Medical Stomatological Academy, Poltava

Chorna Iryna O. MD, PhD, assistant professor,
Department of General Surgery,
Ukrainian Medical Stomatological Academy.
36011 Ukraine, Poltava, Shevchenka Str. 23,
+38(05322)2-68-74,
Irina-ch17@rambler.ru
http://orcid.org/0000-0002-0371-8072

References

Gerstein AD, Phillips TJ, Rogers GS, Gilchrest BA. Wound healing and aging. Dermatol Clin. 1993;11(4):749-57. doi: 10.1016/S0733-8635(18)30227-4. PMID: 8222358.

Ashcroft GS, Ashworth JJ. Potential role of estrogens in wound healing. Am J Clin Dermatol. 2003;4(11):737-43. doi: 10.2165/00128071-200304110-00002. PMID: 14572296.

Calvin M. Oestrogens and wound healing. Maturitas. 2000;34(3):195-210. doi: 10.1016/s0378-5122(99)00079-1. PMID: 10717485.

Gilliver SC, Ashcroft GS. Sex steroids and cutaneous wound healing: the contrasting influences of estrogens and androgens. Climacteric. 2007;10(4):276-88. doi: 10.1080/13697130701456630. PMID: 17653954.

Hardman MJ, Ashcroft GS. Estrogen, not intrinsic aging, is the major regulator of delayed human wound healing in the elderly. Genome Biol. 2008;9(5):R80. doi: 10.1186/gb-2008-9-5-r80. Epub 2008 May 13. PMID: 18477406; PMCID: PMC2441466.

Briskin BS, Puzina SN, Kostyuchenko LN, editors. Surgical diseases in geriatrics. Moscow: BINOM-Press; 2006.336 p. [In Russian]. ISBN 5-9518-0122-2.

Dudnichenko AS, Krasnopolskiy YM, Shvets VI. Liposomal drugs in the expert and clinic. Kharkiv: RA-Caravel; 2001.143 p. [In Russian].

Kreines VM, Melnikova VM, Margolin YM, Melyantseva LP. Anti-inflammatory effects of liposomes. Vestnik AMN SSSR. 1990;(6): 44-7. [In Russian].

Abaev YUK, Surgeon's Handbook: Wounds and wound infection. Rostov on Don: Phoenix; 2006. 427 p. [In Russian]. ISBN 5-222-07691-1.

Kaydashev IP, editor. Methods of clinical and experimental research in medicine. Poltava: Polimet; 2003.320 s. [In Ukrainian].

Published
2020-09-30
How to Cite
LihonenkoО. V., StorozhenkoО. V., ZubakhaА. B., ShumeikoІ. А., & ChornaІ. О. (2020). Complex treatment of experimental purulent wound of soft tissues. Klinicheskaia Khirurgiia, 87(7-8), 58-62. https://doi.org/10.26779/2522-1396.2020.7-8.58
Section
Experimental Investigations