Malabsorption syndrome and changes in concentration of endogenous antimicrobial peptids and cytokines in hepatic echinococcosis
Objective. To study the levels of some cytokines (IL-6, TNF-α) and endogenous antimicrobial peptides (zonulin, calprotectin and lactoferrin) in patients, suffering hepatic echinococcosis, and to follow-up their changes in dynamics of postoperative treatment.
Materials and methods. There were examined 29 patients, ageing 35 - 65 yrs old, operated for hepatic echinococcosis. In accordance to scheme of the treatment conducted after laparoscopic echinococcectomy the patients were distributed into two Groups: the first one - 13 patients, who obtained the standard treatment, and the second - 16 patients, to whom, besides the standard treatment, preparation for immunocorrection was prescribed. Antibodies towards the echinococcus IgG, concentration of endogenous antimicrobal peptides and cytokines in the blood serum were studied, using immuno-enzymal analysis in dynamics.
Results. There was established, that in preoperative period in patients, suffering hepatic echinococcosis, high levels of the studied inflammation mediators are revealed, comparing with the indices in healthy persons. Conduction of postoperative treatment promotes a staged lowering of the antimicrobal peptides and cytokines concentrations. Standard treatment is less effective in lowering of the inflammation mediators levels, than combination of conventional treatment with immunocorrection.
Conclusion. In the patients, suffering hepatic echinococcosis, the levels of cytokines and antimicrobal peptides are raised. Combined treatment with immunocorrection after echinococcusectomy leads to significant lowering of the inflammation mediators levels.
Azizzoda ZA, Kurbonov KM, Rizoev VS. Minimally invasive surgery for the liver echinococcosis. Avicenna Bulletin. 2019;21(1):116-20. Russian. doi.org/10.25005/2074-0581-2019-21-1-116-120.
Amonov ShSh, Rakhmonov DA, Fayziev ZSh, Bokiev FB, Turakulov FA, Sangov DS. Modern aspects of diagnostics and surgical treatment of hepatic echinococcosis. Avicenna Bulletin. 2019;21(3): 480-8. Russian. doi: 10.25005/2074-0581-2019-21-3-480-488.
Mourglia-Ettlin G, Amezcua-Vesely MC, Fraga R, Baz A, Merino MC, Gruppi A, Dematteis S. Echinococcus granulosus glycoconjugates induce peritoneal B cell differentiation into antibody-secreting cells and cytokine production. Parasite Immunol. 2011 Nov;33(11):621-31. doi: 10.1111/j.1365-3024.2011.01326.x. PMID: 21992445.
Vafin AZ, Mashurova EV. Cytokine Expression in the Liver Hydatidosis Patients. Annals of HPB Surgery. 2007.12(4): 32-5. Russian.
Mashurova EV. Cytokine profile in liver echinococcosis. PhD [thesis]. Moskva; 2008. 23 s. Russian.
Mirhodzhaev IA. The role of cytokines in the development and progress of echinococcus of the liver. Biology and integrative medicine. 2020;(2): 62-74. Russian.
Daminova NM, Kurbonov KM. Diagnostics and treatment of postoperative complications of liver echinococcectomy. Healthcare of Tajikistan. 2010;(1):109-13. Russian.
Nasirov MY, Alekberova SA, Abbasalieva PM. The role of cytokines in the pathology of the digestive system. Surgery (Azerbaijan). 2014;(2):86-93. Russian.
Kokryakov VN, Aleshina GM, Shamova OV, Orlov DS, Andreeva YuV. Modern concept of antimicrobial peptides as molecular factor of the immunity. Med Acad J. 2010; 10(4):149-60. Russian.
Kulakova EV, Elizarova VM, Pampura AN. Endogenous antimicrobial polypeptides are factors of nonspecific defense of the organism. Russian dental journal. 2012; (6): 42-5. Russian.
Mondragón-de-la-Peña C, Ramos-Solís S, Barbosa-Cisneros O, Rodríguez-Padilla C, Tavizón-García P, Herrera-Esparza R. Echinococcus granulosus down regulates the hepatic expression of inflammatory cytokines IL-6 and TNF alpha in BALB/c mice. Parasite. 2002 Dec;9(4):351-6. doi: 10.1051/parasite/2002094351. PMID: 12514950.
Ketlinskiy SA, Simbircev AS. Cytokines. SPeterburgb: Foliant; 2008. 552 p. Russian. ISBN: 978-5-93929-171-2.
Chu WM. Tumor necrosis factor. Cancer Lett. 2013 Jan 28;328(2):222-5. doi: 10.1016/j.canlet.2012.10.014. Epub 2012 Oct 22. PMID: 23085193; PMCID: PMC3732748.
Obedin AN, Minaev SV. Zonulin as a Forecasting Marker of Inflammatory Complications after Surgery at Newborns with Congenital Intestinal Tract Development Malformations. Journal of New Medical Technologies. 2011;18(4):83-4. Russian.
Shumatova TA, Zernova ES, Grigoryan LA, Shishatskaya SN. Antimicrobial peptides and proteins in diagnosis of malabsorption syndrome in children. Modern problems of science and education. [Internet]. 2015;(3). Russian.
Available from: http://www.science-education.ru/ru/article/view?id=20324.
Fasano A. Intestinal permeability and its regulation by zonulin: diagnostic and therapeutic implications. Clin Gastroenterol Hepatol. 2012 Oct;10(10):1096-100. doi: 10.1016/j.cgh.2012.08.012. Epub 2012 Aug 16. PMID: 22902773; PMCID: PMC3458511.
Dolgih TI. Fecal calprotectin - non-invasive biomarker inflammatory process of the intestine. Laboratory. 2013;(2):44-6. Russian.
Lazebnik LB, Guseynzade M, Efremov LI, Sagynbaeva VE, Knyazev OV. Fecal calprotectin as a biomarker effectiveness of various interventions in patients with inflammatory bowel disease. Experimental and Clinical Gastroenterology. 2013;(8):11-6. Russian.
Usacheva OV, Pechugina VV. Faecal calprotectin as a diagnostic inflammatory marker of severity in the intestinal mucous membrane (a literature review). Sovremennaya pediatriya. 2018;(3):70-3; Ukrainian. doi: 10.15574/SP.2018.91.70.
Moreno-Expósito L, Illescas-Montes R, Melguizo-Rodríguez L, Ruiz C, Ramos-Torrecillas J, de Luna-Bertos E. Multifunctional capacity and therapeutic potential of lactoferrin. Life Sci. 2018 Feb 15;195:61-64. doi: 10.1016/j.lfs.2018.01.002. Epub 2018 Jan 4. PMID: 29307524.
Siqueiros-Cendón T, Arévalo-Gallegos S, Iglesias-Figueroa BF, García-Montoya IA, Salazar-Martínez J, Rascón-Cruz Q. Immunomodulatory effects of lactoferrin. Acta Pharmacol Sin. 2014 May;35(5):557-66. doi: 10.1038/aps.2013.200. PMID: 24786230; PMCID: PMC4814036.
This work is licensed under a Creative Commons Attribution 4.0 International License.