Blood serum amino acid pool violation of in patients with liver cirrhosis

Keywords: liver cirrhosis; amino acid pool; intestinal dysbiosis.

Abstract

ObjectiveTo investigate serum amino acid pool changes in patients with liver cirrhosis.

Materials and methods. 95 patients with liver cirrhosis were participated in the study. In the 1st group (class A disease severity according to Child- Pugh score) there were 18 (18.95%) patients, in the 2nd group (class B) - 25 (26.3%), in the 3rd groups (class C) - 52 (54.7%) patients. The amount of free amino acids in blood serum was determined by reversed-phase high-performance liquid chromatography in isocratic elution mode with electrochemical detection.

Results. Violations of free amino acid reserves were revealed in patients with cirrhosis of the liver with a predominant accumulation of aromatic amino acids: phenylalanine - (162.45 ± 14.12) nmol/ml, tyrosine - (99.05 ± 10.07) nmol/ml, tryptophan - (76.10 ± 12.40) nmol/ml, as well as an increase in the content of proline, lysine, cysteine (p <0.05) . In parallel, the decreasing of free serum amino acids with branched side chain levels were observed: valine - up (150.10 ± 8.92) nmol/ml leucine - up (75.14 ± 5.12) nmol/ml (p < 0.05) and isoleucine - up to (80.40 ± 8.01) nmol/ml.

Conclusions. The correlation between the second degree of thick guts dysbiosis and increased levels of tryptophan was determined (r = 0.77; p <0.01). III degree thick dysbiosis was correlated with increasing levels of phenylalanine (r = 0.71; p <0.01). In patients of 2nd group the correlations between levels of tryptophan and II degree of intestinal dysbiosis (r = 0.58; p <0.01) and the levels of tyrosine in phenylalanine in III stage intestinal dysbiosis were detected.

Author Biography

O. P. Balazh, Uzhgorod National University

Balazh Olesia P., MD, Anestesiologist
Center Pulmonary Diseases,
Assistant Department of public health and humanitarian disciplines
Medical faculty No 2, Uzhhorod National University
3 Narodna Sqr., 88000 Uzhgorod , Ukraine
+38 099 736 0819
obalazh20@gmail.com
orcid.org/0000-0001-7559-0672

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Published
2021-03-28
How to Cite
Balazh, O. P. (2021). Blood serum amino acid pool violation of in patients with liver cirrhosis. Klinicheskaia Khirurgiia, 88(1-2), 19-22. https://doi.org/10.26779/2522-1396.2021.1-2.19
Section
General Problems of Surgery