Cytokine and Antimicrobial Peptide Levels in Cholelithiasis
Objective. To study the cytokines and antimicrobial peptides concentrations in patients with cholelitiasis during 1 year treatment before and after the surgery.
Materials and methods. 38 patients with a cholelithiasis were examined and operated in the Educational-Surgical Clinic of the Azerbaijan Medical University. According to the treatment regimen after the operation the patients were divided into two groups: patients of the 1st group (n = 17) received standard treatment; patients of 2nd group (n = 21), in addition to standard treatment hepatoprotectors, combined enzyme preparations, pre- and probiotics were prescribed. The control group consisted of 14 apparently healthy individuals. The levels of inflammatory cytokines (interleukin-6 and tumor necrosis factor-α) in the blood and antimicrobial peptides (zonulin, calprotectin, and lactoferrin) in feces we studied with enzyme immunoassay method.
Results. In patients with cholelithiasis of the 1st group, the concentration of interleukin-6 was significantly increased by 5 times (p <0.001), the concentration of tumor necrosis factor-α - by 3.5 times (p <0.001), of zonulin - by 2.2 times (p = 0.012), of calprotectin - by 5.8 times (p <0.001, the concentration of lactoferrin - by 60 times (p <0.001), and in the 2nd group - by 4.8 times (p <0.001), by 3.1 times (p <0.001), on 91.7% (p = 0.011), by 5.8 times (p <0.001) and by 52.8 times (p <0.001) respectively in comparison with the control.
Conclusions. Standard postoperative treatment supplemented by hepatoprotectors, combined enzyme preparations, pre- and probiotics prescription leads to a positive dynamics of inflammatory mediators concentration and its normalization.
2. Shchegolev AA, editor. Cholera: a methodological guide. Moscow: RNIMU; 2015. 35 s
3. Petukhov VA. Gallstone disease and indigestion syndrome. Mocow: Vedi; 2003. 127 p. Russian. ISBN 5-94624-014-5.
4. Keller J, Layer P. The Pathophysiology of Malabsorption. Viszeralmedizin. 2014 Jun;30(3):150-4. doi: 10.1159/000364794. PMID: 26288588; PMCID: PMC4513829.
5. Tsaregorodtseva TM, Serova TI. Cytokines in gastroenterology. - Moscow: Anacharsis; 2003.96 p. Russian. ISBN: 5-901352-30-0.
6. Gadzhiev DN, Tagiev ÉG, Guseĭnaliev AG, Gadzhiev ND, Talyshinskaia LR. The cytokine profile in the patients with acute calculous cholecystitis and correction of its disorders. Klin Khir. 2013 Apr;(4):17-9. Russian. PMID: 23888711.
7. Gadzhiyev JN, Gadzhiyev NJ, Gasymova SK. Endogenic antimicrobial peptides and cytokines in calculous cholecystitis. Khirurgiia (Mosk). 2018;(10):51-4. Russian. doi: 10.17116/hirurgia201810151. PMID: 30531737.
8. Ilyashenko MG. Clinical and diagnostic value of antimicrobial peptides in ulcerative colitis. PhD(Med) [dissertation]. Rostov-on-Don: RSMU; 2014.176 s. Russian.
9. Nasirov MYa, Alekberova SA, Abbasalieva PM. The role of cytokines in the pathology of the digestive system. Surgery (Baku). 2014;(2):86-93. Russian.
10. Ostanin AA, Starostina NM, Meledina IV, Shipunov MV, Leplina OYu, Shevela EY, et al. A multiplex assay of 26 cytokines secreted by blood cells of patients with liver cirrhosis. Medical Immunology (Russia). 2015;17(6):539-52. Russian. doi: 10.15789/1563-0625-2015-6-539-552.
11. Matveev AV. Hepatoprotectors. Analysis of international studies on drugs in the group of drugs for the liver. Simferopol:Arial;2013. 384 p. Russian. ISBN 978-617-648-199-7.
12. Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295. doi: 10.1101/cshperspect.a016295. PMID: 25190079; PMCID: PMC4176007.
13. Chu WM. Tumor necrosis factor. Cancer Lett. 2013 Jan 28;328(2):222-5. doi: 10.1016/j.canlet.2012.10.014. Epub 2012 Oct 22. PMID: 23085193; PMCID: PMC3732748.
14. Fasano A. Intestinal permeability and its regulation by zonulin: diagnostic and therapeutic implications. Clin Gastroenterol Hepatol. 2012 Oct;10(10):1096-100. doi: 10.1016/j.cgh.2012.08.012. Epub 2012 Aug 16. PMID: 22902773; PMCID: PMC3458511.
15. Sturgeon C, Fasano A. Zonulin, a regulator of epithelial and endothelial barrier functions, and its involvement in chronic inflammatory diseases. Tissue Barriers. 2016 Oct 21;4(4):e1251384. doi: 10.1080/21688370.2016.1251384. PMID: 28123927; PMCID: PMC5214347.
16. Lazebnik LB, Guseynzade M, Efremov LI, Sagynbaeva VE, Knyazev OV. Fecal calprotectin as a biomarker effectiveness of various interventions in patients with inflammatory bowel disease. Experimental and Clinical Gastroenterology. 2013;(8):11-6. Russian.
17. Usacheva OV, Pechugina VV. Faecal calprotectin as a diagnostic inflammatory marker of severity in the intestinal mucous membrane (a literature review) sovremennaya pediatriya. 2018;(3):70-3. Ukrainian. doi 10.15574/SP.2018.91.70.
18. Szmyt M, Kasprzak A, Malkowski W, Surdyk-Zasada J, Przybyszewska W, Siodła E, et al. Tissue expression of S100 proteins in gallbladder mucosa of the patients with calculous cholecystitis. Folia Histochem Cytobiol. 2013;51(2):141-8. doi: 10.5603/FHC.2013.0022. PMID: 23907944.
19. Siqueiros-Cendón T, Arévalo-Gallegos S, Iglesias-Figueroa BF, García-Montoya IA, Salazar-Martínez J, Rascón-Cruz Q. Immunomodulatory effects of lactoferrin. Acta Pharmacol Sin. 2014 May;35(5):557-66. doi: 10.1038/aps.2013.200. PMID: 24786230; PMCID: PMC4814036.
20. Lamb CA, Mansfield JC. Measurement of faecal calprotectin and lactoferrin in inflammatory bowel disease. Frontline Gastroenterol. 2011 Jan;2(1):13-8. doi: 10.1136/fg.2010.001362. Epub 2010 Nov 3. PMID: 23904968; PMCID: PMC3724198.
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