Endogenous intoxication indicators in advanced peritonitis and their correction

Keywords: widespread peritonitis; lipid peroxidation; medium molecular peptides.

Abstract

ObjectiveTo study the lipid peroxidation processes and endogenous intoxication indicators in patients with widespread peritonitis before surgery and during the treatment.

Materials and methods. The study included 97 patients (43 men and 54 women) aged from 17 to 69 years with purulent peritonitis. According to treatment scheme, patients were divided into two groups. Patients in group 1 received standard treatment, and in patients in group 2 standard treatment was supplemented by immunoreactions. The concentration of diene conjugates and malonic dialdehyde, reduced glutathione, catalase activity and the concentration of medium molecular weight peptides were determined in the blood of patients and almost healthy people.

Results. The concentration of diene conjugates, malonic dialdehyde and medium molecular weight peptides in the blood of patients with advanced peritonitis increased significantly depending on the clinical stage of the disease, while the activity of the enzyme catalase and the concentration of reduced glutathione were decreased.

Conclusions. Immunoreactions in combination with standard treatment leads to normalization of lipid peroxidation and reduction of endogenous intoxication on the 5th and 10th days of treatment.

Author Biography

E. T. Mammadova, Azerbaijan Medical University, Baku

E. T. Mammadova, Azerbaijani Medical University, Baku
Mammadova Esmira Tarverdi, MD.
Chief laboratory of department of surgical diseases III.
General surgeon of Surgical clinic of Azerbaijan Medical University
Surgical clinic of Azerbaijan Medical University.
Azerbaijan Medical University.
155 Samed Vurgun Str., Baku, Azerbaijan
+994 51 923 09 23
mesmira1412@gmail.com
https://orcid.org/0000-0001-7503-8917

References

Styazhkina SN, Ovechkina IA, Shakirova LC, Khabibullina GF. Peritonitis in modern abdominal surgery. International scientific review. 2017;(4):98-102. Russian.

Hall JC, Heel KA, Papadimitriou JM, Platell C. The pathobiology of peritonitis. Gastroenterology. 1998 Jan;114(1):185-96. doi: 10.1016/s0016-5085(98)70646-8. PMID: 9428232.

Hasanov MD. Algorithm for assessing the severity of endotosycosis in peritonitis. Bulletin of the Russian Military Medical Academy. 2014;(4):75-8. Russian.

Gadzhiev NJ. Immune status, cytokine profile and endogenous intoxication in patients with diffuse peritonitis. Surgery of Ukraine. 2012;(4):67-75. Russian.

Vlasov AP, Timoshkin SP, Abramova SV, Vlasov PA, Shibitov VA, Polozova EI. Indicators of endogenous intoxication - criteria of enteral insufficiency in acute peritonitis. Fundamental Research. 2014;(10-60:1066-70. Russian.

Dalbaşı E, Gedik E, Tüzün A, Obay BD. Correlation of Malondialdehyde and Antioxidant Enzyme Levels with Peritonitis Severity in Patients with Generalized Peritonitis. Dicle Med J. 2020;47(2):293-303. DOI: 10.5798/dicletip.

Matveyev SB, Fedorova NV, Godkov MA. Evaluation of endogenous intoxication from average molecular weight peptides in emergencies. Klinicheskaya Laboratornaya Diagnostika. 2009 May;(5):16-8. Russian.

Vlasov AP, Abramova SV, Vlasov PA, Timoshkin SP, Leshchankina NY, Kochetkova TA, et al. Evaluation of enteric insufficiency in terms of lipid peroxidation. Modern problems of science and education. 2014;(6):1101. Russian.

Blinkov YU, Sukovatykh BS, Stroyev YUS, Konoplya AI. Characteristics of immune and oxidative disorders in patients with widespread fibrinous-purulent peritonitis. Man and His Health (Kursk). 2010;(1):64-70. Russian.

Savytskyi IV, Znamerovsky SG, Dzygal OF. The role of lipid peroxidation in the pathogenesis of experimental peritonitis. Journal of Education, Health and Sport. 2020;10(7):389-95. doi: 10.12775/JEHS.2020.10.07.041.

Ermola YuA. Changes in indicators of lipid peroxidation and antioxidant system in the development of experimental peritonitis. Pathology. 2012;(2):92-4. Russian.

Gavrilov VB, Gavrilova AR, Khmara NF. Measurement of diene conjugates in blood plasma using the UV absorption of heptane and isopropanol extracts. Lab Delo. 1988;(2):60-4. Russian. PMID: 2452294.

Andreeva LI, Kozhemiakin LA, Kishkun AA. Modification of the method of determining lipid peroxidation in a test using thiobarbituric acid. Lab Delo. 1988;(11):41-3. Russian. PMID: 2463416.

Nikolaychik VV, Moin VM, KirkovskiyVV, Mazur LI, Lobacheva GA, Bychko GN, et al. A method of determining middle molecules. Lab Delo. 1991;(10):13-8. Russian. PMID: 1722520.

Kumar Y, Singh G, Davidson BR. Free radical and antioxidant levels in patients with secondary peritonitis and their prognostic significance. Dig Surg. 2007;24(5):331-7. doi: 10.1159/000106511. Epub 2007 Jul 27. PMID: 17664875.

Bezruchko NV, Rubtsov GK, Ganyayeva NB, Kozlova GA, Sadovnikova DG. Catalase of biological environments of the human body and its clinical biochemical value in endotoxicose estimation. Tomsk State Pedagogical University Bulletin. 2012;(7):94-9. Russian.

Published
2021-03-28
Section
General Problems of Surgery