Improving the effectiveness of the skin integrity restoring after deep burns by platelet-enriched plasma application

Keywords: burns; platelet-enriched plasma; wound healing; autodermoplasty.

Abstract

ObjectiveTo study the effectiveness of platelet-enriched plasma application in patients with deep burns at the stage of wounds preparation for autodermoplasty.

Materials and methods. The study was performed on 23 patients with burns IIA, IIB and III degrees taking 25 - 40% of the body surface. Comprehensive local treatment of patients of the main group included injection of platelet-enriched plasma at the stage of preparation of postoperative wounds for autodermoplasty. In patients of the control group, local treatment was limited by dressings with antiseptic solution. Examination of patients included visual inspection of the damaged area, microbiological examination of wound contents and histological examination of damaged tissues.

Results. Applying the suggested method of burn wounds local treatment resulted in reducing time of wounds preparation for closure by autologous skin grafts, reducing of their healing period, decreasing of tissues inflammatory activity, blood flow improvement, and stimulating processes of tissue granulation, proliferation, epithelialization.

Conclusions. The application of the autologous platelet-enriched plasma can be considered as an effective biotechnology that can increase the effectiveness of the skin integrity restoring in patients with burns.

Author Biographies

R. M. Chornopyshchuk, National Pirogov Memorial Medical University, Vinnytsya

Chornopyshchuk Roman Mykolaiovych- MD, PhD(Med), Assistant Professor
Department of General Surgery,
National Pirogov Memorial Medical University
56 Pirogov str., Vinnytsya, 21028, Ukraine,
+38(0432)55-39-10,
r.chornopyshchuk@gmail.com
https://orcid.org/0000-0001-5422-7495.

V. I. Nagaichuk, National Pirogov Memorial Medical University, Vinnytsya

Nagaichuk Vasyl Ivanovych - MD, Sci(Med), Professor
Department of General Surgery,
National Pirogov Memorial Medical University
56 Pirogov str., Vinnytsya, 21028, Ukraine,
+38(0432)55-39-10,
vasyl.nagaychuk@gmail.com
https://orcid.org/0000-0001-9238-9276.

O. A. Nazarchuk, National Pirogov Memorial Medical University, Vinnytsya

Nazarchuk Oleksandr Adamovych - MD, Sci(Med), Professor
Department of General Surgery,
National Pirogov Memorial Medical University
56 Pirogov str., Vinnytsya, 21028, Ukraine,
+38(0432)55-39-10, nazarchukoa@gmail.com
https://orcid.org/0000-0001-7581-0938.

M. D. Zheliba, National Pirogov Memorial Medical University, Vinnytsya

Zheliba Mykola Dmytrovych - MD, Sci(Med), Professor
Department of General Surgery,
National Pirogov Memorial Medical University
56 Pirogov str., Vinnytsya, 21028, Ukraine,
+38(0432)55-39-10,m.d.zheliba@gmail.com
https://orcid.org/0000-0002-3233-553X.

O. G. Urvan, Vinnytsya Regional Pathological and Anatomical Bureau

Urvan Olena Heorhiivna - MD,
Main department of general pathology with histological researches
Vinnytsya Regional Pathological and Anatomical Bureau
46 Pirogov str., Vinnytsya, 21028, Ukraine
+38(0432)67-00-68
vinnitsa_opik_17@ukr.net
https://orcid.org/0000-0001-5269-6722.

References

Oryan A, Alemzadeh E, Moshiri A. Burn wound healing: present concepts, treatment strategies and future directions. J Wound Care. 2017 Jan 2;26(1):5-19. doi: 10.12968/jowc.2017.26.1.5. PMID: 28103165.

Tejiram S, Romanowski KS, Palmieri TL. Initial management of severe burn injury. Curr Opin Crit Care. 2019 Dec;25(6):647-52. doi: 10.1097/MCC.0000000000000662. PMID: 31567292.

Zuo H, Song G, Shi W, Jia J, Zhang Y. Observation of viable alloskin vs xenoskin grafted onto subcutaneous tissue wounds after tangential excision in massive burns. Burns Trauma. 2016 May 27;4:23. doi: 10.1186/s41038-016-0045-9. PMID: 27574692; PMCID: PMC4964051.

Nagaichuk VI, Khimich SD, Zheliba MD, Zhuchenko OP, Povoroznik AM, Prysyazhnyuk MB, et al. Modern technologies of treatment of patients with critical and supercritical burns. Reports of Vinnytsia National Medical University [Internet]. 2017;21(2):428-33. Ukrainian. Available from: http://irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?C21COM=2&I21DBN=UJRN&P21DBN=UJRN&IMAGE_FILE_DOWNLOAD=1&Image_file_name=PDF/vvnmu_2017_21_2_14.pdf.

Alekseev AA, Salakhiddinov KZ, Gavrilyuk BK, Tyurnikov YuI. Complex treatment of deep burns on basis of surgical necrectomies and modern biotechnological methods. Annaly Khirurgii. 2012;(6):41-5. Russian.

Maranda EL, Rodriguez-Menocal L, Badiavas EV. Role of Mesenchymal Stem Cells in Dermal Repair in Burns and Diabetic Wounds. Curr Stem Cell Res Ther. 2017;12(1):61-70. doi: 10.2174/1574888x11666160714115926. PMID: 27412677.

Gupta S, Goil P, Thakurani S. Autologous Platelet Rich Plasma As A Preparative for Resurfacing Burn Wounds with Split Thickness Skin Grafts. World J Plast Surg. 2020 Jan;9(1):29-32. doi: 10.29252/wjps.9.1.29. PMID: 32190588; PMCID: PMC7068187.

Farroha A. Effects of COVID-19 pandemic on burns epidemiology. Burns. 2020 Sep;46(6):1466. doi: 10.1016/j.burns.2020.05.022. Epub 2020 May 29. PMID: 32507521; PMCID: PMC7256614.

Lumenta DB, Kamolz LP, Frey M. Adult burn patients with more than 60% TBSA involved-Meek and other techniques to overcome restricted skin harvest availability--the Viennese Concept. J Burn Care Res. 2009 Mar-Apr;30(2):231-42. doi: 10.1097/BCR.0b013e318198a2d6. PMID: 19165111.

Marck RE, Gardien KL, Stekelenburg CM, Vehmeijer M, Baas D, Tuinebreijer WE, et al. The application of platelet-rich plasma in the treatment of deep dermal burns: A randomized, double-blind, intra-patient controlled study. Wound Repair Regen. 2016 Jul;24(4):712-20. doi: 10.1111/wrr.12443. Epub 2016 Jun 3. PMID: 27169627.

Tsai HC, Chang GR, Fan HC, Ou-Yang H, Huang LC, Wu SC, et al. A mini-pig model for evaluating the efficacy of autologous platelet patches on induced acute full thickness wound healing. BMC Vet Res. 2019 Jun 7;15(1):191. doi: 10.1186/s12917-019-1932-7. PMID: 31174527; PMCID: PMC6556007.

Malanga GA, Goldin M. PRP: review of the current evidence for musculoskeletal conditions. Curr Phys Med Rehabil Rep. 2014;2:1-15. doi: 10.1007/s40141-013-0039-5

Nazarchuk OA, Chereshniuk IL, Nazarchuk HH. The research of antimicrobial efficacy of antiseptics decamethoxin, miramistin and their effect on nuclear DNA fragmentation and epithelial cell cycle. Wiad Lek. 2019;72(3):374-80. PMID: 31050983.

Huang SH, Wu SH, Lee SS, Lin YN, Chai CY, Lai CS, et al. Platelet-Rich Plasma Injection in Burn Scar Areas Alleviates Neuropathic Scar Pain. Int J Med Sci. 2018 Jan 8;15(3):238-47. doi: 10.7150/ijms.22563. PMID: 29483815; PMCID: PMC5820853.

Published
2021-03-28
How to Cite
Chornopyshchuk, R. M., Nagaichuk, V. I., Nazarchuk, O. A., Zheliba, M. D., & Urvan, O. G. (2021). Improving the effectiveness of the skin integrity restoring after deep burns by platelet-enriched plasma application. Klinicheskaia Khirurgiia, 88(1-2), 50-56. https://doi.org/10.26779/2522-1396.2021.1-2.50
Section
General Problems of Surgery