Experimental investigations of application of the water-steam оzone-containing gaseous mixture for prophylaxis and treatment of respiratory infectious diseases

Keywords: the water-steam ozone-containing mixture; polyetiological respiratory diseases; virus of transmissive gastroenteritis of pigs; family of coronaviruses.

Abstract

Objective. Determination of the virus-cidal and bacterio-cidal action of the ozone-containing steam-water mixture, which was obtained, using apparatus POS-1, simulated on viral models of transmissive gastroenteritis of pigs of the coronaviruses family and polyresistant clinical strains of bacterial cultures, as well as adjustment of optimal parameters of ozone in the gaseous mixture content, which do not cause pathological changes in the organism’s organs and systems.

Materials and methods. Apparatus POS-1, created by collective of Scientific-Methodical Centre «Medical Innovation Technologies»», was applied for production of the ozone-containing gaseous mixture. Virusological investigations were conducted on the base of the Institute of Epidemiology and Infectious Diseases named after L. V, Gromashevskiy. As a working material the models of virus of the pigs transmissive gastroenteritis of the coronaviruses family were applied. The series of bacteriological and experimental investigations were conducted on the base of Shalimov National Institute of Surgery and Transplantology NAMS of Ukraine. Polyresistant cultures in concentration of 108 colony-creating units per 1 ml were applied for bacteriological investigations. The experiment was conducted on white rats to study the gaseous ozone-containing mixture affection on living biological tissues.

Results. Virusological investigations have shown, that in the 20 minutes exposition and a contact with extracellular virus the infection titer have lowered in 100 000 times, and in a 30 minutes exposition - a complete deactivation of virus have had occurred. Bacteriological investigations have revealed the complete absence of the cultures development after their processing with the ozone-containing mixture during 20 min. The results of swimming tests and behavioral reactions in white rats of control and experimental groups did not differ. Histological investigations of the respiratory organs tissues as well as of spleen, thyroid gland, kidneys and suprarenal glands did not reveal pathological effects of the ozone-containing mixture.

Conclusion. Parameters of the ozone generation and delivery in the gaseous mixture content were studied and optimally selected. Investigations of the ozone-containing mixture affection on a viral strain of the coronaviruses family, as well as bacteriological investigations on polyresistant bacterial cultures have had confirmed its pronounced virus-cidal and bactericidal properties. Application of the ozone-containing mixture do not cause any pathological changes in the living organism organs and systems. This gaseous mixture may be used for prevention and treatment of respiratory infectious diseases of viral and bacterial genesis.

Author Biographies

M. V. Kostylev, Shalimov National Institute of Surgery and Transplantology, Kyiv

Kostylev М.V., MD, DSci(Med), Professor
Shalimov National Institute of Surgery and Transplantology,
30 Geroyev Sevastopoliya str., Kyiv, Ukraine, 03126;
mkv.via@gmail.com,
https://orcid.org/0000-0002-2559-4058.

S. L. Rybalko, Gromashevskiy Institute of Epidemiology and Infectious Diseases, Kyiv

Rybalko SL., MD, DSci(Med), Professor
Gromashevskiy Institute of Epidemiology and Infectious Diseases

5 N.Amosova str., Kiev, Ukraine, 03038;
y_dasha@ukr.net,
http://orcid.org/0000-0002-1913-1380.

A. A. Vladimirov, Shupyk National Healthcare University of Ukraine, Kyiv

Vladimirov A. A., - MD, DSci(Med), professor
Head of the Department of Physical and Rehabilitation Medicine and sports medicine
Shupyk National Healthcare University of Ukraine, Kyiv;
9, Dorogozhitskaya str., Kiev, 04112, Ukraine,
avladimirov05@gmail.com;
https://orcid.org/0000-0001-5106-6382

N. V. Chukhraiev, Scientific-Methodical Centre «Medical Innovation Technologies», Kyiv

Chukhraiev M. V., DSci(Med),
Head of MEDINTECH, (Kyiv
office 60, Pochayninska53 str., Kyiv, Ukraine, 04070,
nv1111155@gmail.com;
https://orcid.org/0000-0001-5290-8887

G. V. Terehov, Shalimov National Institute of Surgery and Transplantology, Kyiv

Terehov G. V, MD, PhD(Med),
Head of the Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantology,
30 Geroyev Sevastopoliya str., Kyiv, Ukraine, 03126;
doctorterehov@gmail.com; tel+380679311431.
https://orcid.org/000-0002-7625-9778

S. N. Titarenko, Shalimov National Institute of Surgery and Transplantology, Kyiv

Titarenko S. M, bacteriologist
Shalimov National Institute of Surgery and Transplantology,
30 Geroyev Sevastopoliya str., Kyiv, Ukraine, 03126;
dr.titarenko@ukr.net,
+380661536518,
https://orcid.org/0000-0001-5656-2254

D. B. Starosila, Gromashevskiy Institute of Epidemiology and Infectious Diseases, Kyiv

Starosila D. B., MD, PhD(Med), Senior Researcher,
Gromashevskiy Institute of Epidemiology and Infectious Diseases
3, N.Amosova str., Kiev, Ukraine, 03038;
y_dasha@ukr.net,

https://orcid.org/0000-0003-0210-2361.

I. M. Savytska, Shalimov National Institute of Surgery and Transplantology, Kyiv

Savytska I. M., MD, PhD(Med), senior researcher,
Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantology,
30 Geroyev Sevastopoliya str., Kyiv, Ukraine, 03126;
irinasavitska@ukr.net,+380975100401,
https://orcid.org/0000-0020-2749-7825

O. A. Geylenko, Shalimov National Institute of Surgery and Transplantology, Kyiv

Geylenko O. A., researcher
Department of Experimental Surgery,
Shalimov National Institute of Surgery and Transplantology,
30 Geroyev Sevastopoliya str., Kyiv, Ukraine, 03126;
ogeylenko@ukr.net;
https://orcid.org/0000-0001-5342-3881.

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Published
2021-10-28
How to Cite
Kostylev, M. V., Rybalko, S. L., Vladimirov, A. A., Chukhraiev, N. V., Terehov, G. V., Titarenko, S. N., Starosila, D. B., Savytska, I. M., & Geylenko, O. A. (2021). Experimental investigations of application of the water-steam оzone-containing gaseous mixture for prophylaxis and treatment of respiratory infectious diseases. Klinicheskaia Khirurgiia, 88(5-6), 69-74. https://doi.org/10.26779/2522-1396.2021.5-6.69
Section
Experimental Investigations