Possibilities of clinical application of nano-crystal hydroxyapatyte in treatment of purulent-inflammatory diseases

Keywords: experimental peritonitis; composite materials; sorption-aspiration drainage system.

Abstract

Objective. To investigate the impact of the sorbtion-aspiration drainage system on background of hydroxyapatite and zinc oxide on experimental peritonitis.

Materials and methods. The experiment was performed on 55 white laboratory rat males, ageing 2 - 3 mo, with the body mass 225 - 250 g, which were kept in the typical vivarium conditions. Suspension of E. coli in physiological solution, 1.0 - 1.5 ml in concentration 1010 , was used for the peritoneum contamination. The suspension was introduced into abdominal cavity in the dose, dependent on the animal body mass, using the syringe for insulin injection. Peritonitis in the rats was developed in 48 h and characterized by intoxication, which exaggerated rapidly, as well as by intestinal motor disorders and pronounced microcirculatory disturbances. The experimental animals were distributed into 5 Groups. In patients of Group I the interventions were not performed (control); in laboratory animals of Group II peritonitis was not simulated, but the abdominal cavity was drained, using the sorption-aspiration drainage system; in laboratory animals of Group III peritonitis was simulated; in the Group IV of laboratory animals on background of peritonitis the abdominal cavity was drained, using the sorption-aspiration drainage system; in the Group V animals the abdominal cavity was drained by a standard silicon drain on background of peritonitis.

Results. Reduction of abdominal contamination by 43.12% and of histological features were produced in abdominal organs under the influence of the composite system, witnessing its bactericidal and sorption-antiinflammatory action.

Conclusion. Experimentally there was confirmed the possibility of application of sorption-aspiration drainage system on background of hydroxyapatite and zinc oxide in treatment of an acute peritonitis.

Author Biographies

І. D. Duzhyi, Sumy State University

Duzhyi Igor, MD, PhD, DSci(Med),
Head of the Department of Surgery, traumatology, orthopedics and phthisiolog
Sumy State University.
48 Troitska str., 40000, Sumy, Ukraine
+380990081259
gensurgery@med.sumdu.edu.ua
http://orcid.org/0000-0002-4995-0096

L. F. Sukhodub, Sumy State University

Sukhodub Leonid, DSci(Phys-Mathem), Professor,
Head of the Department of Biophysics, Biochemistry, Pharmacology and Biomolecular Engineering
Sumy State University.
2 Rymskogo-Korsakova st., 40007 Sumy, Ukraine
+380503077859
l.sukhodub@med.sumdu.edu.ua
http://orcid.org/0000-0002-1559-0475

V. S. Bielai, Sumy State University

Bielai Volodymyr, postgraduate
Department of Surgery, traumatology, orthopedics and phthisiology
Sumy State University
1/1 Zalivnaya st. 25, 40024 Sumy, Ukraine
+380663749166
vlbelay@gmail.com
https://orcid.org/0000-0002-7909-3064

O. M. Lytvynenko, Sumy State University

Oleksandr Lytvynenko, MD, DSci(Med), Professor
Department of surgery, traumatology, orthopedics and phthisiology
Sumy State University.
2, Rymskogo-Korsakova st., 40007 Sumy, Ukraine
+380506225577,
omlytvynenko@gmail.com
https://orcid.org/0000-0003-0806-5047

A. V. Yurchenko, Sumy State University

Yurchenko Alla, MD, PhD(Med), Assistant Professor
Department of Neurosurgery and Neurology with Courses of Psychiatry, Narcology, Medical Psychology, Occupational diseases, Section of Orthopedics and Traumatology.
Sumy State University, Ukraine.
48 Troitska str., 40000, Sumy, Ukraine
+380509771346
a.yurchenko@med.sumdu.edu.ua
https://orcid.org/0000-0002-9707-8829

References

Buyanov VM, Rodoman GV, Laberko LA. Surgical peritonitis. From a unified classification to a unified treatment strategy. Ross Med J.1998;(4):3-10. Russian.

Tsarapkin IM, Pleshakov VT, Bessmeltsev SS. Rheological properties of peripheral blood in acute diffuse purulent peritonitis and their changes during infusion therapy with the use of a modegel. Grekov's Bulletin of Surgery. 1999;158(6):72-5. Russian.

Bonkemeyer Millan S, Gan R, Townsend PE. Venous Ulcers: Diagnosis and Treatment. Am Fam Physician. 2019 Sep 1;100(5):298-305. PMID: 31478635.

Jull AB, Cullum N, Dumville JC, Westby MJ, Deshpande S, Walker N. Honey as a topical treatment for wounds. Cochrane Database Syst Rev. 2015 Mar 6;(3):CD005083. doi: 10.1002/14651858.CD005083.pub4. PMID: 25742878.

Vashchuk VV, Kirik TP, Kushnirchuk MI, Baidala RP, Ivanishin AZ, Gritsenko OM. A new technique for local therapy of trophic ulcers of the lower extremities. Kharkivska khirurhichna shkola. 2018;(1):51-3. Ukrainian.

O'Meara S, Richardson R, Lipsky BA. Topical and systemic antimicrobial therapy for venous leg ulcers. JAMA. 2014 Jun 25;311(24):2534-5. doi: 10.1001/jama.2014.4574. PMID: 25058087.

Norman G, Westby MJ, Rithalia AD, Stubbs N, Soares MO, Dumville JC. Dressings and topical agents for treating venous leg ulcers. Cochrane Database Syst Rev. 2018 Jun 15;6(6):CD012583. doi: 10.1002/14651858.CD012583.pub2. PMID: 29906322; PMCID: PMC6513558.

Sukhodub LB, Sukhodub LF, Prylutskyy YuI, Strutynska NYu, Vovchenko LL, Soroca VM, et al. Composite material based on hydroxyapatite and multi-walled carbon nanotubes filled by iron: Preparation, properties and drug release ability. Materials Science & Engineering C. 2018 Dec 01;93:606-14. doi: 10.1016/j.msec.2018.08.019.

Pogrebnjak A, Sukhodub L, Sukhodub L, Bondar O, Kumeda M, Shaimardanova B, et al. Composite material with nanoscale architecture based on bioapatite, sodium alginate and ZnO microparticles. 2019;45(6):7504-14. doi: 10.1016/j.ceramint.2019.01.043.

Published
2021-10-28
How to Cite
DuzhyiІ. D., Sukhodub, L. F., Bielai, V. S., Lytvynenko, O. M., & Yurchenko, A. V. (2021). Possibilities of clinical application of nano-crystal hydroxyapatyte in treatment of purulent-inflammatory diseases. Klinicheskaia Khirurgiia, 88(5-6), 75-80. https://doi.org/10.26779/2522-1396.2021.5-6.75
Section
Experimental Investigations