Еxperimental investigation of morphological changes in pancreatic gland after its resection, using electrosurgical instruments

  • O. Yu. Usenko Shupyk National Medical Academy of Postgraduate Education, Kyiv, Shalimov National Institute of Surgery and Transplantology, Kyiv https://orcid.org/0000-0003-4957-4104
  • K. G. Valikhnovska Shupyk National Medical Academy of Postgraduate Education, Kyiv https://orcid.org/0000-0001-8287-0736
  • I. M. Savytska Shalimov National Institute of Surgery and Transplantology, Kyiv
  • V. V. Likhodiyevskyi Bogomolets National Medical University, Kyiv
Keywords: pancreatic resection; еlectric welding; coagulation necrosis; еlectrosurgery; bipolar welding; monopolar welding

Abstract

Objective. To determine a peculiarities of morphological changes in pancreatic gland after resectional operations, using the high-energy electric surgical instruments (HEESI) and standard surgical procedure.

Маterials and methods. In conditions of experiment a pancreatic resection was performed in white laboratory rats, using a standard surgical procedure and a high-energy electro-surgical instruments (HEESI) in a welding regime (DA2), bipolar and monopolar regimes. Histological investigation of pancreatic gland was done immediately postoperatively and in 3, 7 and 21 days, using a light-optical microscope Leica DМ500.

Results. In early terms after application of a standard surgical procedure a more severe inflammation have had occur and more rapidly the newly created connective tissue volume enhanced, than after application of electro-surgical methods. After all kinds of electro-resection performed in a newly created tissue the ducts have been formatted, what was not observed after application of a standard procedure. After bipolar welding the surface resected have had lesser hematomas, than after application of surgical scissors. After resection, performed, using monopolar welding, a less dense coagulation layer have been created, than after resection, performed with application of a bipolar welding, in 21 days in the resection zone a significantly more volume of connective tissue was created and more significant signs of inflammation persisted, than after application of a bipolar method.

Conclusion. While applying of all kinds of electro-resection the operative intervention duration shortens, the hemorrhage occurrence risk reduces, hermetic joints on the surface resected are created, and the restoration processes do not complicate.

Author Biographies

O. Yu. Usenko, Shupyk National Medical Academy of Postgraduate Education, Kyiv, Shalimov National Institute of Surgery and Transplantology, Kyiv

Usenko Olexander Yu - MD. Dr.Sc. Professor,

Director of the O.O. Shalimov National Institute of Surgery and Transplantology

Address: 30 Heroiv Sevastopolia Str., 03680, Kyiv, Ukraine,

 tel.: +38 (044)4971374

usenko1962@gmail.com

https://orcid.org/0000-0003-4957-4104

K. G. Valikhnovska, Shupyk National Medical Academy of Postgraduate Education, Kyiv

Valikhnovska KG,

postgraduate of Surgery and Transplantology Dpt.,

Shupyk National Academy of Postgraduate Education,

9 Dorohozhytska Str., Kyiv, 04112

+38-044) 408-19-90

E-mail:Kgv.112@gmail.com,

https://orcid.org/0000-0001-8287-0736

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Published
2018-10-29
Section
Experimental Investigations